ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection

• Published in: Journal of neuroimmunology Vol. 213; no. 1; pp. 12 - 19
• Main Authors: Tse, Margaret C.L, Lane, Crystal, Mott, Kevin, Onlamoon, Nattawat, Hsiao, Hui-Mien, Perng, Guey Chuen
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18-08-2009
• Subjects: Allergy and Immunology; Animals; Brain - immunology; Brain - metabolism; Brain - virology; Cells, Cultured; Chemokines; Chemokines - metabolism; Chemokines - secretion; Cytokines - metabolism; Cytokines - secretion; Disease Models, Animal; Down-Regulation - immunology; Encephalitis, Herpes Simplex - immunology; Encephalitis, Herpes Simplex - metabolism; Encephalitis, Herpes Simplex - physiopathology; Female; Herpes simplex virus 1; Herpesvirus 1, Human - immunology; Herpesvirus 1, Human - metabolism; HSE; HSV-1; ICAM-5; Immunologic Factors - metabolism; Interleukins - metabolism; Interleukins - secretion; Lymphocyte Activation - immunology; Lymphocyte Count; Lymphocytes - immunology; Lymphocytes - metabolism; Lymphocytes - secretion; Membrane Glycoproteins - metabolism; Mice; Nerve Tissue Proteins - metabolism; Neurological disorder; Neurology; Up-Regulation - immunology; Viral Load; Virulence Factors - metabolism; HSV-1; HSE; Neurological disorder; ICAM-5; Chemokines
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2009.06.007

Abstract Chemokines are important in HSE development in the CNS but underlying regulatory events are unknown. Two-hybrid binding assays identified that intercellular adhesion molecule 5 (ICAM-5), an immune modulator in the CNS, interacted with neurovirulence factor, UOL, of HSV-1. Viral load and interleukin levels were similar in UOL deletion virus (ΔUOL), and wild type virus infected mouse brains. However, higher numbers of lymphocytes, but unaltered soluble ICAM-5 and chemokine levels were detected in ΔUOL infected mouse brains. In contrast, lower lymphocyte numbers, reduced soluble ICAM-5, and higher chemokine levels were detected in wild type virus infected brains. Our results suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.