A C4-oxidizing Lytic Polysaccharide Monooxygenase Cleaving Both Cellulose and Cello-oligosaccharides

• Published in: The Journal of biological chemistry Vol. 289; no. 5; pp. 2632 - 2642
• Main Authors: Isaksen, Trine, Westereng, Bjørge, Aachmann, Finn L., Agger, Jane W., Kracher, Daniel, Kittl, Roman, Ludwig, Roland, Haltrich, Dietmar, Eijsink, Vincent G.H., Horn, Svein J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 31-01-2014; American Society for Biochemistry and Molecular Biology
• Subjects: AA10; AA9; Biofuel; Biofuels - microbiology; Carbon - metabolism; CBM33; Cellulase; Cellulose - metabolism; Enzymology; GH61; Lytic Polysaccharide Monooxygenase (LPMO); Mass Spectrometry; Mass Spectrometry (MS); Metalloenzymes; Mixed Function Oxygenases - metabolism; Neurospora crassa - enzymology; Neurospora crassa - metabolism; NMR; Oligosaccharides - metabolism; Oxidation-Reduction; Oxygen - metabolism; Polysaccharides - metabolism; AA10; CBM33; NMR; Cellulase; Mass Spectrometry (MS); AA9; Biofuel; Lytic Polysaccharide Monooxygenase (LPMO); Metalloenzymes; GH61
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M113.530196

Lignocellulosic biomass is a renewable resource that significantly can substitute fossil resources for the production of fuels, chemicals, and materials. Efficient saccharification of this biomass to fermentable sugars will be a key technology in future biorefineries. Traditionally, saccharification was thought to be accomplished by mixtures of hydrolytic enzymes. However, recently it has been shown that lytic polysaccharide monooxygenases (LPMOs) contribute to this process by catalyzing oxidative cleavage of insoluble polysaccharides utilizing a mechanism involving molecular oxygen and an electron donor. These enzymes thus represent novel tools for the saccharification of plant biomass. Most characterized LPMOs, including all reported bacterial LPMOs, form aldonic acids, i.e., products oxidized in the C1 position of the terminal sugar. Oxidation at other positions has been observed, and there has been some debate concerning the nature of this position (C4 or C6). In this study, we have characterized an LPMO from Neurospora crassa (NcLPMO9C; also known as NCU02916 and NcGH61–3). Remarkably, and in contrast to all previously characterized LPMOs, which are active only on polysaccharides, NcLPMO9C is able to cleave soluble cello-oligosaccharides as short as a tetramer, a property that allowed detailed product analysis. Using mass spectrometry and NMR, we show that the cello-oligosaccharide products released by this enzyme contain a C4 gemdiol/keto group at the nonreducing end.
Background: Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that cleave polysaccharides.
Results: We describe a novel LPMO and use a range of analytical methods to characterize its activity.
Conclusion: Cellulose and cello-oligosaccharides are cleaved by oxidizing the sugar at the nonreducing end in the C4 position.
Significance: This study provides unequivocal evidence for C4 oxidation of the nonreducing end sugar and demonstrates a novel LPMO substrate specificity.