Bile Acids Trigger GLP-1 Release Predominantly by Accessing Basolaterally Located G Protein–Coupled Bile Acid Receptors

• Published in: Endocrinology (Philadelphia) Vol. 156; no. 11; pp. 3961 - 3970
• Main Authors: Brighton, Cheryl A, Rievaj, Juraj, Kuhre, Rune E, Glass, Leslie L, Schoonjans, Kristina, Holst, Jens J, Gribble, Fiona M, Reimann, Frank
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01-11-2015; Oxford University Press
• Subjects: Acids; Animals; Bile; Bile acids; Bile Acids and Salts - pharmacology; Calcium (intracellular); Calcium - metabolism; Calcium ions; Cell membranes; Cells, Cultured; Cyclic AMP - metabolism; Digestive system; Enteroendocrine Cells - drug effects; Enteroendocrine Cells - metabolism; Fluorescence; Gastrointestinal tract; Glucagon; Glucagon-like peptide 1; Glucagon-Like Peptide 1 - metabolism; Intestine; Intestine, Small - drug effects; Intestine, Small - metabolism; Intracellular Space - drug effects; Intracellular Space - metabolism; Male; Mice, Knockout; Mice, Transgenic; Microscopy, Fluorescence; Original Research; Proteins; Rats, Wistar; Receptors; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Secretion; Sensors; Taurodeoxycholic Acid - pharmacology; Taurolithocholate; Taurolithocholic Acid - pharmacology; Tissue Culture Techniques; Transgenic mice
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2015-1321

Bile acids are well-recognized stimuli of glucagon-like peptide-1 (GLP-1) secretion. This action has been attributed to activation of the G protein–coupled bile acid receptor GPBAR1 (TGR5), although other potential bile acid sensors include the nuclear farnesoid receptor and the apical sodium-coupled bile acid transporter ASBT. The aim of this study was to identify pathways important for GLP-1 release and to determine whether bile acids target their receptors on GLP-1–secreting L-cells from the apical or basolateral compartment. Using transgenic mice expressing fluorescent sensors specifically in L-cells, we observed that taurodeoxycholate (TDCA) and taurolithocholate (TLCA) increased intracellular cAMP and Ca2+. In primary intestinal cultures, TDCA was a more potent GLP-1 secretagogue than taurocholate (TCA) and TLCA, correlating with a stronger Ca2+ response to TDCA. Using small-volume Ussing chambers optimized for measuring GLP-1 secretion, we found that both a GPBAR1 agonist and TDCA stimulated GLP-1 release better when applied from the basolateral than from the luminal direction and that luminal TDCA was ineffective when intestinal tissue was pretreated with an ASBT inhibitor. ASBT inhibition had no significant effect in nonpolarized primary cultures. Studies in the perfused rat gut confirmed that vascularly administered TDCA was more effective than luminal TDCA. Intestinal primary cultures and Ussing chamber–mounted tissues from GPBAR1-knockout mice did not secrete GLP-1 in response to either TLCA or TDCA. We conclude that the action of bile acids on GLP-1 secretion is predominantly mediated by GPBAR1 located on the basolateral L-cell membrane, suggesting that stimulation of gut hormone secretion may include postabsorptive mechanisms.