Platelet-Rich Fibrin-Conditioned Medium as an Alternative to Fetal Bovine Serum Promotes Osteogenesis of Human Dental Pulp Stem Cells

• Published in: Bioengineering (Basel) Vol. 10; no. 10; p. 1196
• Main Authors: Hatori, Ayano, Yamakawa, Daiki, Al-Maawi, Sarah, Dohle, Eva, Chikira, Jin, Fujii, Yasuyuki, Miki, Megumu, Sader, Robert, Chikazu, Daichi, Ghanaati, Shahram, Kawase-Koga, Yoko
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-10-2023; MDPI
• Subjects: Anticoagulants; Bioengineering; Biomaterials; Biomedical materials; Blood; Blood platelets; Calcification; Cell culture; Cell differentiation; Cell growth; Cell proliferation; Consent; Culture; Culture media; Cytology; Dental pulp; Differentiation (biology); Fetal calf serum; Fibrin; Gene expression; Growth factors; human dental pulp stem cells; Leukocytes; Medical research; Mesenchymal stem cells; Observations; Osteoblastogenesis; Osteogenesis; osteogenic differentiation; Pathogens; Physical characteristics; Physiological aspects; platelet-rich fibrin; Platelets; Stem cells; tissue engineering; xeno-free medium; Japan; St Louis Missouri; France; United States > US
• ISSN: 2306-5354; 2306-5354
• DOI: 10.3390/bioengineering10101196

Human dental pulp stem cells (DPSCs) exhibit multilineage differentiation capabilities and superior clonogenic and proliferative properties. However, the use of animal-derived components such as FBS raises concerns regarding the clinical application of stem-cell-based therapies. Platelet-rich fibrin (PRF) derived from human blood is rich in fibrin, platelets, and growth factors and acts as a bioactive scaffold for grafting with biomaterials. In this study, we assessed the efficacy of PRF-conditioned medium (CM) in promoting DPSCs proliferation and osteogenic differentiation compared with the standard culture medium supplemented with FBS. A comparison of DPSCs cultured in FBS and PRF-CM revealed no differences in characteristics or morphology. However, cells cultured with PRF-CM exhibited inferior proliferation rates and cell numbers during passage in comparison with those cultured with FBS. In contrast, DPSCs cultured in PRF-CM showed significantly higher levels of calcification, and RT-PCR confirmed that the gene expression levels of markers associated with osteoblast differentiation were significantly increased. The PRF-CM approach offers a convenient, straightforward, and advantageous method for culturing DPSCs, without relying on animal-derived components. In summary, this study introduces a novel application of PRF-CM for enhancing the osteogenesis of DPSCs, which provides an alternative to FBS culture medium and addresses concerns associated with the use of animal-derived components in clinical settings.