Fine-scale clonal structure and diversity within patches of a clone-forming dioecious shrub, Ilex leucoclada (Aquifoliaceae)

• Published in: Annals of botany Vol. 95; no. 2; pp. 295 - 304
• Main Authors: Torimaru, T, Tomaru, N
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-01-2005; Oxford Publishing Limited (England)
• Subjects: clonal diversity; clonal growth; clonal structure; clonal variation; Demography; dioecy; Ecological genetics; female flowers; Forest ecology; genet; Genetic diversity; genetic markers; Genetic Variation - genetics; Ilex; Ilex - genetics; Ilex leucoclada; layering; male flowers; Marine ecology; Models, Genetic; Original; patch; Phenotype; Phenotypes; phenotypic variation; Plants; Population ecology; Population genetics; ramet; Random Amplified Polymorphic DNA Technique; RAPD; seedling recruitment; shrubs; spatial distribution; Species diversity; Stems; Japan
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mci025

• Background and Aims The mode of reproduction (sexual vs. asexual) is likely to have important effects on genetic variation and its spatial distribution within plant populations. An investigation was undertaken of fine-scale clonal structure and diversity within patches of Ilex leucoclada (a clone-forming dioecious shrub). • Methods Six patches were selected in a 1-ha plot previously established in an old-growth beech forest. Two of the selected patches were composed predominantly of stems with male flowers (male patch), and two contained stems with predominantly female flowers (female patch). The remaining two patches contained stems with male flowers and stems with female flowers in more or less equal proportions (mixed patch). Different genets were distinguished using random amplified polymorphic DNA (RAPD) markers. • Key Results One hundred and fifty-six genets with different RAPD phenotypes were identified among 1928 stems from the six patches. Among the six patches, the male patches had the lowest clonal diversity, and the mixed patches had the highest. Distribution maps of the genets showed that they extended downhill, reflecting natural layering that occurred when stems were pressed to the ground by heavy snow. In every patch, there were a few large genets with many stems and many small genets with a few stems. • Conclusion The differences in clonal diversity among patches may be due to differences in seedling recruitment frequencies. The skewed distribution of genet size (defined as the number of stems per genet) within patches may be due to differences in the timing of germination, or age (with early-establishing genets having clear advantages for acquiring resources) and/or intraspecific competition.