The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis

• Published in: Nature communications Vol. 6; no. 1; p. 6095
• Main Authors: Saito, Hikaru, Oikawa, Takaya, Hamamoto, Shin, Ishimaru, Yasuhiro, Kanamori-Sato, Miyu, Sasaki-Sekimoto, Yuko, Utsumi, Tomoya, Chen, Jing, Kanno, Yuri, Masuda, Shinji, Kamiya, Yuji, Seo, Mitsunori, Uozumi, Nobuyuki, Ueda, Minoru, Ohta, Hiroyuki
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04-02-2015; Nature Publishing Group; Nature Pub. Group
• Subjects: 13; 38; 631/1647/334/2244/710; 631/449/1741; 631/449/2653; 631/449/2675; 82; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Cyclopentanes - metabolism; Flowers - growth & development; Flowers - metabolism; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; Gibberellins - metabolism; Humanities and Social Sciences; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; multidisciplinary; Oxylipins - metabolism; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms7095

Plant hormones are transported across cell membranes during various physiological events. Recent identification of abscisic acid and strigolactone transporters suggests that transport of various plant hormones across membranes does not occur by simple diffusion but requires transporter proteins that are strictly regulated during development. Here, we report that a major glucosinolate transporter, GTR1/NPF2.10, is multifunctional and may be involved in hormone transport in
Arabidopsis thaliana
. When heterologously expressed in oocytes, GTR1 transports jasmonoyl-isoleucine and gibberellin in addition to glucosinolates.
gtr1
mutants are severely impaired in filament elongation and anther dehiscence resulting in reduced fertility, but these phenotypes can be rescued by gibberellin treatment. These results suggest that GTR1 may be a multifunctional transporter for the structurally distinct compounds glucosinolates, jasmonoyl-isoleucine and gibberellin, and may positively regulate stamen development by mediating gibberellin supply.
GTR1 is known to transport glucosinolates in
Arabidopsis
. Here, Saito
et al
. show that GTR1 also transports the plant hormones jasmonate and gibberellin when heterologously expressed in
Xenopus
oocytes, and that
gtr1
mutant plants show a gibberellin-related fertility phenotype.