Effects of puerarin on the AKT signaling pathway in bovine preadipocyte differentiation

• Published in: Animal bioscience Vol. 33; no. 1; pp. 4 - 11
• Main Authors: Yun, Jinyan, Yu, Yongsheng, Zhou, Guoli, Luo, Xiaotong, Jin, Haiguo, Zhao, Yumin, Cao, Yang
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01-01-2020; Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST); Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: adipogenesis; Adipose tissue; akt signaling pathway; Anopheles; Beef cattle; Biomedical laboratory equipment; bovine; Cattle; Genes; Genetic research; Messenger RNA; Polymerase chain reaction; preadipocyte differentiation; Protein binding; puerarin; RNA; Scientific equipment industry; Time; Triglycerides; 축산학; United States; AKT Signaling Pathway; Bovine; Puerarin; Preadipocyte Differentiation; Adipogenesis
• ISSN: 1011-2367; 2765-0189; 1976-5517; 2765-0235
• DOI: 10.5713/ajas.19.0004

Puerarin has the potential of regulating the differentiation of preadipocytes, but its mechanism of action has not yet been elucidated. Adipocytes found in adipose tissue, the main endocrine organ, are the main sites of lipid deposition, and are widely used as a cell model in the study of in vitro fat deposition. This study aimed to investigate the effects of puerarin on adipogenesis in vitro.
Puerarin was added to the culture medium during the process of adipogenesis. The proliferation and differentiation of bovine preadipocytes was measured through cell viability and staining with Oil Red O. The content of triacylglycerol (TG) was measured using a triglyceride assay kit. The mRNA and protein expression levels of adipogenic genes, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α (C/EBPα), were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively.
The addition of puerarin significantly increased adipogenesis of bovine preadipocytes and enhanced the mRNA and protein level expression of PPARγ (p<0.01). The expression of P-Akt increased after adipogenic hormonal induction, whereas puerarin significantly increased PPARγ expression by promoting the Akt signaling component, P-Akt. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473, which may activate the downstream signaling of the Akt pathway.
Puerarin was able to promote the differentiation of preadipocytes and improve fat deposition in cattle. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473.