Expression of intercellular adhesion molecule-1 by myofibers in mdx mice

• Published in: Muscle & nerve Vol. 52; no. 5; pp. 795 - 802
• Main Authors: Torres-Palsa, Maria J., Koziol, Matthew V., Goh, Qingnian, Cicinelli, Peter A., Peterson, Jennifer M., Pizza, Francis X.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-11-2015; Wiley Subscription Services, Inc
• Subjects: Adhesion; Animals; Duchenne muscular dystrophy; Intercellular Adhesion Molecule-1 - biosynthesis; macrophages; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Skeletal - pathology; muscle inflammation; Muscle, Skeletal - metabolism; Muscle, Skeletal - pathology; Muscular dystrophy; Muscular Dystrophy, Duchenne - diagnosis; Muscular Dystrophy, Duchenne - metabolism; neutrophils; Rodents; β2 integrins; macrophages; β2 integrins; muscle inflammation; neutrophils; Duchenne muscular dystrophy
• ISSN: 0148-639X; 1097-4598
• DOI: 10.1002/mus.24626

ABSTRACT
Introduction: We investigated the extent to which intercellular adhesion molecule‐1 (ICAM‐1), a critical protein of the inflammatory response, is expressed in skeletal muscles of mdx mice (a murine model of Duchenne muscular dystrophy). Methods: Muscles were collected from control and mdx mice at 2‐24 weeks of age and analyzed for ICAM‐1 expression by means of Western blot and immunofluorescence. Results: Western blot revealed higher expression of ICAM‐1 in mdx compared with control muscles through 24 weeks of age. In contrast to control muscles, ICAM‐1 was expressed on the membrane of damaged, regenerating, and normal myofibers of mdx mice. CD11b+ myeloid cells also expressed ICAM‐1 in mdx muscles, and CD11b+ cells were closely associated with the membrane of myofibers expressing ICAM‐1. Conclusions: These findings support a paradigm in which ICAM‐1 and its localization to myofibers in muscles of mdx mice contributes to the dystrophic pathology. Muscle Nerve 52: 795–802, 2015