Analytical Performance Evaluation of the New GEM ® Premier™ 5000 in Comparison to the Epoc ® Blood Gas Analyzer in Horses

• Published in: Veterinary sciences Vol. 10; no. 2; p. 114
• Main Authors: Sandersen, Charlotte, Dmitrovic, Petra, Dupont, Julien, Cesarini, Carla, Guyot, Hugues, Serteyn, Didier, Kirsch, Katharina
• Format: Journal Article Web Resource
• Language: English
• Published: Switzerland MDPI AG 03-02-2023; MDPI
• Subjects: Agreements; Analysis; Analytical instruments; Bias; Bland–Altman analysis; blood gas analysis; Blood gases; Correlation analysis; Electrolytes; Gases; General anesthesia; General Veterinary; Hemoglobin; horse; Horses; Laboratories; Life sciences; method comparison; Médecine vétérinaire & santé animale; Passing–Bablok analysis; Regression analysis; Sciences du vivant; Statistical analysis; Veterinary medicine & animal health; Belgium; horse; Passing–Bablok analysis; method comparison; blood gas analysis; Bland–Altman analysis
• ISSN: 2306-7381; 2306-7381
• DOI: 10.3390/vetsci10020114

Different blood gas analyzers are used in equine practice. Every machine needs to be validated, as they have not been designed for use in horses. The aim of this study was to compare the newly marketed GEM5000 machine to the formerly validated epoc machine for blood gas analysis in horses. In this prospective, non-blinded, comparative laboratory analyzer study, 43 equine blood samples were analyzed on both analyzers and values were compared between the two machines via Lin's concordance analysis, Passing-Bablok regression analysis and Bland-Altman plots. Duplicate measurements were conducted on the GEM5000 machine to evaluate precision. The GEM5000 failed to achieve the required precision for tHb, Hct and iCa
, but achieved acceptable precision for all other parameters. Concordance correlation analysis revealed poor correlation for Na
, Cl
, iCa
, K
, Hct and tHb, while there was an at least moderate agreement for all other parameters. Passing-Bablok regression revealed significant constant bias for pCO
, pO
, Cl
, and iCa
and significant proportional bias for pCO
, iCa
and SO
. Bland-Altman analysis revealed significant systematic bias for Na
, Cl
, iCa
, K
, Hct, tHb and SO
. This study shows that while precision of the GEM5000 is good, values should not be used interchangeably with data obtained from other blood gas analyzers.