Sporothrix schenckii COMPLEX:SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 57; no. 4; pp. 289 - 294
• Main Authors: Oliveira, Daniele Carvalho, de Loreto, Érico Silva, Mario, Débora Alves Nunes, Lopes, Paulo G Markus, Neves, Louise Vignolles, da Rocha, Marta Pires, Santurio, Janio Morais, Alves, Sydney Hartz
• Format: Journal Article
• Language: English
• Published: Brazil Instituto de Medicina Tropical de Sao Paulo 01-07-2015; Instituto de Medicina Tropical; Universidade de São Paulo (USP)
• Subjects: Animals; Antifungal agents; Antifungal Agents - pharmacology; Cats; Combination therapy; Enzymatic profile; Humans; Itraconazole - pharmacology; Ketoconazole - pharmacology; Microbial Sensitivity Tests; Mycology; Naphthalenes - pharmacology; Phylogeny; Sporothrix; Sporothrix - drug effects; Sporothrix - enzymology; Susceptibility; TROPICAL MEDICINE; Voriconazole - pharmacology; Sporothrix; Enzymatic profile; Combination therapy; Susceptibility; Antifungal agents
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46652015000400003

Sporothrix schenckii was reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans(n = 1) , S. brasiliensis(n = 6) , S. globosa(n = 1), S. mexicana(n = 1) and S. schenckii(n = 36) to terbinafine (TRB) alone and in combination with itraconazole (ITZ), ketoconazole (KTZ), and voriconazole (VRZ) by a checkerboard microdilution method and determined the enzymatic profile of these species with the API-ZYM kit. Most interactions were additive (27.5%, 32.5% and 5%) or indifferent (70%, 50% and 52.5%) for TRB+KTZ, TRB+ITZ and TRB+VRZ, respectively. Antagonisms were observed in 42.5% of isolates for the TRB+VRZ combination. Based on enzymatic profiling, the Sporothrix schenckii strains were categorized into 14 biotypes. Leucine arylamidase (LA) activity was observed only for S. albicans and S. mexicana. The species S. globosa and S. Mexicana were the only species without β-glucosidase (GS) activity. Our results may contribute to a better understanding of virulence and resistance among species of the genus Sporothrix in further studies.