In vitro activity of Amazon plant extracts against Enterococcus faecalis

• Published in: Brazilian journal of microbiology Vol. 45; no. 3; pp. 769 - 779
• Main Authors: de Castilho, Adriana Lígia, da Silva, Juliana Paola Correa, Saraceni, Cintia Helena Coury, Díaz, Ingrit Elida Collantes, Paciencia, Mateus Luís Barradas, Varella, Antonio Drauzio, Suffredini, Ivana Barbosa
• Format: Journal Article
• Language: English
• Published: Brazil Springer Nature B.V 01-09-2014; Sociedade Brasileira de Microbiologia
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Bacteria; Brazil; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Enterococcus faecalis - drug effects; Herbal medicine; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; MICROBIOLOGY; Models, Molecular; Molecular Structure; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plants - chemistry; Rainforests; Research Paper; Studies; Brazil; Enterococcus faecalis; antibacterial activity; Amazon rain forest; bioprospection; endodontitis
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1590/s1517-83822014000300002

Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests' plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC(®) 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 μg/mL), Diclinanona calycina (MIC ≤ 40 μg/mL) and Moronobea coccinea (40 < MIC < 80 μg/mL; MBC = 80 μg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by (13)C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.