Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms

• Published in: Marine environmental research Vol. 54; no. 3-5; pp. 553 - 557
• Main Authors: Viant, Mark R, Pincetich, Christopher A, Walton, Jeffrey H, Tjeerdema, Ronald S, Hinton, David E
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01-09-2002; Elsevier
• Subjects: Abalone; Adenosine Triphosphate - analysis; Animal, plant and microbial ecology; Animals; Applied ecology; ATP; Biological and medical sciences; Copper; Copper - adverse effects; Development; Ecotoxicology, biological effects of pollution; Embryo, Nonmammalian - physiology; Energy Metabolism; Fundamental and applied biological sciences. Psychology; Haliotis rufescens; In vivo; Magnetic Resonance Spectroscopy - methods; Marine; Medaka; Mollusca - physiology; NMR; Oryzias - embryology; Oryzias - physiology; Oryzias latipes; Phosphates - analysis; Phosphorus; Sublethal stress; Techniques; Water Pollutants - adverse effects; In vivo; NMR; Energy metabolism; Abalone; Medaka; Development; Phosphorus; Sublethal stress; Copper; ATP; Phosphates; Disease; Haliotis rufescens; Interspecific comparison; Gastropoda; Carcinogenesis; Stress; Heavy metal; Aquatic environment; Vertebrata; Sublethal dose; Spectrometry; Pisces; Phosphagen; Non invasive method; Oryzias latipes; Nuclear magnetic resonance; Invertebrata; Mollusca
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/S0141-1136(02)00193-9

In vivo nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for characterizing the sublethal actions of physical and chemical stressors in live, intact organisms. In particular, 31P NMR is ideal for observing perturbations to cellular energetics since critical metabolite concentrations, including phosphagens, ATP and inorganic phosphate (Pi), can be measured non-invasively and in real time. This technique's versatility is demonstrated not only in the diversity of organisms that can be studied, but also in its broad-ranging applicability to environmental research. Illustrative studies include the actions of copper in adult red abalone (Haliotis rufescens) and changes in energetically important metabolites in developing medaka embryos (Oryzias latipes). Advantages and disadvantages of in vivo NMR will be discussed.