Development and validation of a HPTLC method for simultaneous quantitation of flunarizine dihydrochloride and propranolol hydrochloride in capsule dosage form

• Published in: Indian journal of pharmaceutical sciences Vol. 75; no. 3; pp. 364 - 368
• Main Authors: Shivarkar, N, Dudhe, P, Nagras, M
• Format: Journal Article
• Language: English
• Published: India Medknow Publications 01-05-2013; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: Acids; Angina pectoris; Calibration; Chromatography; Drug dosages; Flunarizine; Headaches; Methods; Migraine; Pharmaceuticals; Pharmacology; Propranolol hydrochloride; Short Communications; Standard deviation; Studies; Testing; Thin layer chromatography; India; Mumbai India; high performance thin layer chromatography; Flunarizine dihydrochloride and propranolol hydrochloride; validation
• ISSN: 0250-474X; 1998-3743
• DOI: 10.4103/0250-474X.117418

A simple, precise, accurate, and rapid high-performance thin layer chromatographic method has been developed and validated for the simultaneous quantitation of flunarizine dihydrochloride and propranolol hydrochloride in a combined capsule dosage form. The method was carried out on precoated silica gel 60 F 254 TLC aluminum plate, (20×10 cm 2 ). The solvent system was ethyl acetate:methanol:glacial acetic acid in the proportion of 8:1:1, (v/v/v). Rf value for flunarizine dihydrochloride and propranolol hydrochloride was found to be 0.62±0.02 and 0.18±0.02, respectively. The linearity regression analysis for calibration showed 0.999 and 0.999 for flunarizine dihydrochloride and propranolol hydrochloride with respect to peak area and height in the concentration range of 50-350 ng/spot and 500-3500 ng/spot, respectively. Accuracy of recovery studies was found to be 98-100.28 and 99.11-99.45% for flunarizine dihydrochloride and propranolol hydrochloride, respectively. The amounts of drug in marketed formulation were 100.5 and 101.25% of flunarizine dihydrochloride and propranolol hydrochloride, respectively. The method developed can be used for routine analysis in bulk drug and capsule dosage form.