Super-Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 10; no. 9; pp. 1712 - 1718
• Main Authors: Yang, Hui, Moullan, Norman, Auwerx, Johan, Gijs, Martin A. M.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-05-2014; Wiley Subscription Services, Inc
• Subjects: Animals; Cell Line; Computer Simulation; Diffraction; Doxycycline - pharmacology; Finite Element Analysis; fluorescence; Imaging; Imaging, Three-Dimensional - instrumentation; Immersion; Mice; Microscopes; Microscopy; Microscopy, Fluorescence - methods; microsphere nanoscope; Microspheres; Nanostructure; Nanotechnology; Nanotechnology - instrumentation; Organelles; super resolution; User-Computer Interface
• ISSN: 1613-6810; 1613-6829
• DOI: 10.1002/smll.201302942

Super‐resolution imaging of sub‐cellular organelles and nanostructures is achieved by simply putting high‐refractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's near‐field nanoscopic features into the far‐field, generating a magnified virtual image and demonstrating a resolution four times better than that corresponding to the classical diffraction limit.