Novel platinum-palladium bimetallic nanoparticles synthesized by Dioscorea bulbifera: anticancer and antioxidant activities

• Published in: International journal of nanomedicine Vol. 10; no. default; pp. 7477 - 7490
• Main Authors: Ghosh, Sougata, Nitnavare, Rahul, Dewle, Ankush, Tomar, Geetanjali B, Chippalkatti, Rohan, More, Piyush, Kitture, Rohini, Kale, Sangeeta, Bellare, Jayesh, Chopade, Balu A
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01-01-2015; Dove Medical Press
• Subjects: Analysis; anticancer; Antineoplastic Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - pharmacology; Cell Proliferation - drug effects; Chemical properties; Dioscorea - chemistry; Dioscorea bulbifera; Flow Cytometry; Health aspects; HeLa Cells; Humans; Hydroxyl Radical - chemistry; Metal Nanoparticles - administration & dosage; Metal Nanoparticles - chemistry; Microscopy, Electron, Transmission; Nanoparticles; Nitric Oxide - chemistry; Original Research; Oxidation-Reduction; Palladium; Palladium - chemistry; palladium nanoparticles; Physiological aspects; Plant Extracts - chemistry; Plant Extracts - pharmacology; Platinum; Platinum - chemistry; platinum nanoparticles; platinum-palladium bimetallic nanoparticles; Spectrometry, X-Ray Emission; Superoxides - chemistry; India; palladium nanoparticles; platinum nanoparticles; Dioscorea bulbifera; antioxidant; platinum-palladium bimetallic nanoparticles; anticancer
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S91579

Medicinal plants serve as rich sources of diverse bioactive phytochemicals that might even take part in bioreduction and stabilization of phytogenic nanoparticles with immense therapeutic properties. Herein, we report for the first time the rapid efficient synthesis of novel platinum-palladium bimetallic nanoparticles (Pt-PdNPs) along with individual platinum (PtNPs) and palladium (PdNPs) nanoparticles using a medicinal plant, Dioscorea bulbifera tuber extract (DBTE). High-resolution transmission electron microscopy revealed monodispersed PtNPs of size 2-5 nm, while PdNPs and Pt-PdNPs between 10 and 25 nm. Energy dispersive spectroscopy analysis confirmed 30.88% ± 1.73% elemental Pt and 68.96% ± 1.48% elemental Pd in the bimetallic nanoparticles. Fourier transform infrared spectra indicated strong peaks at 3,373 cm(-1), attributed to hydroxyl group of polyphenolic compounds in DBTE that might play a key role in bioreduction in addition to the sharp peaks at 2,937, 1,647, 1,518, and 1,024 cm(-1), associated with C-H stretching, N-H bending in primary amines, N-O stretching in nitro group, and C-C stretch, respectively. Anticancer activity against HeLa cells showed that Pt-PdNPs exhibited more pronounced cell death of 74.25% compared to individual PtNPs (12.6%) or PdNPs (33.15%). Further, Pt-PdNPs showed an enhanced scavenging activity against 2,2-diphenyl-1-picrylhydrazyl, superoxide, nitric oxide, and hydroxyl radicals.