Simultaneous determination of ten illegal azo dyes in feed by ultra-high performance liquid chromatography tandem mass spectrometry

• Published in: Journal of veterinary research Vol. 61; no. 3; pp. 299 - 305
• Main Authors: Piątkowska, Marta, Jedziniak, Piotr, Olejnik, Małgorzata, Pietruk, Konrad, Żmudzki, Jan, Posyniak, Andrzej
• Format: Journal Article
• Language: English
• Published: Poland De Gruyter Open 01-09-2017; De Gruyter Poland; Sciendo
• Subjects: Acetonitrile; Azo dyes; Chromatography; feed; Formic acid; High-performance liquid chromatography; illegal dyes; Ions; LC-MS/MS; Mass spectrometry; Mass spectroscopy; Scientific imaging; Sudan dyes; Sudan; LC-MS/MS; feed; Sudan dyes; azo dyes; illegal dyes
• ISSN: 2450-7393; 2450-8608; 2450-8608
• DOI: 10.1515/jvetres-2017-0040

Introduction: The paper presents the method of simultaneous determination of 10 illegal azo dyes in feed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry technique. Material and Methods: The dyes were extracted with hexane, evaporated to dryness, and analysed. Separation was achieved in 7 min in a gradient elution using acetonitrile (A) and 0.1% formic acid (B) as a mobile phase. Results: The validation results showed the repeatability of the method, which was evaluated at three levels (50, 500, and 5,000 μg/kg). All the matrix calibration curves for the working ranges were linear (R
0.9904 to 1.0), the repeatability was between 2.1% and 24%, and recoveries ranged from 77.9% to 120%. The LOD and LOQ were at 1-2 and 5-10 μg/kg for different dyes, respectively. Furthermore, the method was applied in the homogeneity tests of the in-house prepared feed containing Sudan I at the levels of 0.5, 5, and 50 mg/kg. Conclusions: A sensitive, selective, and fast multiresidue method was successfully developed and validated. Its robustness was confirmed by the analysis of an experimental feed containing Sudan I.