Whole-cell biosensor of cellobiose and application to wood decay detection

• Published in: Journal of biotechnology Vol. 239; pp. 39 - 46
• Main Authors: Toussaint, Maxime, Bontemps, Cyril, Besserer, Arnaud, Hotel, Laurence, Gérardin, Philippe, Leblond, Pierre
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10-12-2016; Elsevier
• Subjects: Bacteria; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Byproducts; CebR; Cellobiose; Cellobiose - analysis; Cellobiose - metabolism; Computer Simulation; Decay; Degradation; Diagnostic software; Fungi; Fungi - metabolism; Life Sciences; Streptomyces; Streptomyces - metabolism; Synthetic biology; Wood; Wood - chemistry; Wood decay; Streptomyces; Wood decay; Synthetic biology; Cellobiose; CebR; Biosensor
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2016.10.003

•The whole-cell biosensor specifically detects cellobiose.•The biosensor is a fast and powerful tool to detect fungal wood degradation.•Colorimetric detection is based on a catechol dioxygenase reporter gene under control of the engineered Streptomyces CebR master regulator.
Fungal biodegradation of wood is one of the main threats regarding its use as a material. So far, the detection of this decaying process is empirically assessed by loss of mass, when the fungal attack is advanced and woody structure already damaged. Being able to detect fungal attack on wood in earlier steps is thus of special interest for the wood economy. In this aim, we designed here a new diagnostic tool for wood degradation detection based on the bacterial whole-cell biosensor technology. It was designed in diverting the soil bacteria Streptomyces CebR sensor system devoted to cellobiose detection, a cellulolytic degradation by-product emitted by lignolytic fungi since the onset of wood decaying process. The conserved regulation scheme of the CebR system among Streptomyces allowed constructing a molecular tool easily transferable in different strains or species and enabling the screen for optimal host strains for cellobiose detection. Assays are performed in microplates using one-day culture lysates. Diagnostic is performed within one hour by a spectrophotometric measuring of the cathecol deshydrogenase activity. The selected biosensor was able to detect specifically cellobiose at concentrations similar to those measured in decaying wood and in a spruce leachate attacked by a lignolytic fungus, indicating a high potential of applicability to detect ongoing wood decay process.