Single-cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenesis

• Published in: eLife Vol. 10
• Main Authors: Cebrian-Silla, Arantxa, Nascimento, Marcos Assis, Redmond, Stephanie A, Mansky, Benjamin, Wu, David, Obernier, Kirsten, Romero Rodriguez, Ricardo, Gonzalez-Granero, Susana, García-Verdugo, Jose Manuel, Lim, Daniel A, Álvarez-Buylla, Arturo
• Format: Journal Article
• Language: English
• Published: Cambridge eLife Science Publications, Ltd 14-07-2021; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: adult neurogenesis; Analysis; B cells; Bar codes; Brain damage; Embryonic development; Females; Gene expression; Immunocytochemistry; Localization; Neural stem cells; neuroblast; Neurogenesis; Neurons; Neuroscience; Olfactory bulb; Progenitor cells; regional differences; RNA; single-cell sequencing; stem cell; Stem Cells and Regenerative Medicine; Subventricular zone; Ventricle; Ventricle (lateral); Canada
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.67436

The ventricular-subventricular zone (V-SVZ), on the walls of the lateral ventricles, harbors the largest neurogenic niche in the adult mouse brain. Previous work has shown that neural stem/progenitor cells (NSPCs) in different locations within the V-SVZ produce different subtypes of new neurons for the olfactory bulb. The molecular signatures that underlie this regional heterogeneity remain largely unknown. Here, we present a single-cell RNA-sequencing dataset of the adult mouse V-SVZ revealing two populations of NSPCs that reside in largely non-overlapping domains in either the dorsal or ventral V-SVZ. These regional differences in gene expression were further validated using a single-nucleus RNA-sequencing reference dataset of regionally microdissected domains of the V-SVZ and by immunocytochemistry and RNAscope localization. We also identify two subpopulations of young neurons that have gene expression profiles consistent with a dorsal or ventral origin. Interestingly, a subset of genes are dynamically expressed, but maintained, in the ventral or dorsal lineages. The study provides novel markers and territories to understand the region-specific regulation of adult neurogenesis.