Metabolic engineering for production of beta-carotene and lycopene in Saccharomyces cerevisiae

• Published in: Bioscience, biotechnology, and biochemistry Vol. 58; no. 6; pp. 1112 - 1114
• Main Authors: Yamano, S. (Kirin Brewery Co. Ltd., Yokohama (Japan)), Ishii, T, Nakagawa, M, Ikenaga, H, Misawa, N
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1994; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: beta Carotene; Bioconversions. Hemisynthesis; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biotechnology; CAROTENOIDE; CAROTENOIDES; Carotenoids - biosynthesis; Cloning, Molecular - methods; Ergosterol - biosynthesis; Erwinia - genetics; Erwinia - metabolism; Fundamental and applied biological sciences. Psychology; Genetic Engineering - methods; Lycopene; Methods. Procedures. Technologies; Plasmids; Promoter Regions, Genetic; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Terminator Regions, Genetic; VIA BIOQUIMICA DEL METABOLISMO; VOIE BIOCHIMIQUE DU METABOLISME; Heterologous system; Yeast; Genetic transformation; Biosynthesis; Gene expression; Fungi; Gene; Production; Microorganism culture; Bacteria; Ascomycetes; Carotenoid; Secondary metabolism; Saccharomyces cerevisiae; Enterobacteriaceae; Thallophyta
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.58.1112

We have engineered a conventional yeast, Saccharomyces cerevisiae, to confer a novel biosynthetic pathway for the production of beta-carotene and lycopene by introducing the bacterial carotenoid biosynthesis genes, which are individually surrounded by the promoters and terminators derived from S. cerevisiae. The Beta-carotene and lycopene accumulated in the cells of this yeast, which was considered to be a result of the carbon flow for the ergosterol biosynthetic pathway being partially directed to the pathway for the carotenoid production