SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis

• Published in: Molecular plant Vol. 9; no. 7; pp. 1051 - 1065
• Main Authors: Berriri, Souha, Gangappa, Sreeramaiah N., Kumar, S. Vinod
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 06-07-2016; Oxford University Press
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Chromatin Assembly and Disassembly - genetics; Chromatin Assembly and Disassembly - physiology; chromatin remodeling; c亚基; Gene Expression Regulation, Plant; gene regulation; H2A; H2A.Z; Histones - genetics; Histones - metabolism; immunity; Plant Immunity - genetics; Plant Immunity - physiology; SWR1; 免疫效应; 基因调控; 复合物; 拟南芥; 染色质重塑; chromatin remodeling; immunity; SWR1; gene regulation; H2A.Z; arabidopsis
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1016/j.molp.2016.04.003

Incorporation of the histone variant H2A.Z into nucleosomes by the SWR1 chromatin remodeling complex is a critical step in eukaryotic gene regulation. In Arabidopsis, SWRlc and H2A.Z have been shown to con- trol gene expression underlying development and environmental responses. Although they have been implicated in defense, the specific roles of the complex subunits and H2A.Z in immunity are not well under- stood. In this study, we analyzed the roles of the SWRlc subunits, PHOTOPERIOD-INDEPENDENT EARLY FLOWERING1 （PIE1）, ACTIN-RELATED PROTEIN6 （ARP6）, and SWR1 COMPLEX 6 （SWC6）, as well as H2A.Z, in defense and gene regulation. We found that SWRlc components play different roles in resistance to different pathogens. Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance, while loss of ARP6 fucntion resulted in enhanced resistance. We found that mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity. Mutation in SWRlc components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity. Genome-wide expres- sion analyses similarly reveal distinct roles for H2A.Z and SWRlc components in gene regulation, and sug- gest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways. Our data show that although they are part of the same complex, Arabidopsis SWRlc components could have non-redundant functions in plant immunity and gene regulation.