Resveratrol protects neuronal cells from isoflurane-induced inflammation and oxidative stress-associated death by attenuating apoptosis via Akt/p38 MAPK signaling

• Published in: Experimental and therapeutic medicine Vol. 15; no. 2; pp. 1568 - 1573
• Main Authors: Hu, Weilan, Yang, Ei, Ye, Jianxin, Han, Weili, Du, Zeng-Li
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01-02-2018; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Analysis; Anesthesia; Animal cognition; Apoptosis; Biotechnology; Cell cycle; Cytokines; Dosage and administration; Inflammation; Kinases; Laboratories; Lungs; Oxidative stress; Proteins; Pulmonary arteries; Resveratrol; Signal transduction; Tumor necrosis factor; Tumor necrosis factor-TNF; United States > US; China; Germany; neuronal cells; isoflurane; inflammation; resveratrol; oxidative stress
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2017.5527

The aim of the present study was to determine whether resveratrol protects neuronal cells from inflammation and isoflurane-induced oxidative stress-associated death via attenuating apoptosis via Akt/p38 mitogen-activated protein kinase (MAPK) signaling. The PC12 rat pheochromocytoma cell line was treated with 2% isoflurane + 21% O
+ 5% CO
for 6 h and pre-treated with resveratrol (0-1,000 µM) for 0, 24 or 48 h prior to isoflurane treatment. An MTT assay, flow cytometry and ELISA of tumor necrosis factor-α, interleukin-6, malondialdehyde and superoxide dismutase revealed that resveratrol reduced growth inhibition, restrained apoptosis and suppressed inflammation and oxidative stress induced by isoflurane in PC12 cells. Pretreatment with resveratrol effectively reduced caspase-3 activity and inducible nitric oxide synthase protein expression in isoflurane-induced PC12 cells. In addition, western blot analysis demonstrated that resveratrol treatment significantly attenuated isoflurane-induced decreases in the activated phosphorylated (p)-Akt/Akt ratio and increases in the p-p38/p38 MAPK protein ratio in PC12 cells. These findings indicated that resveratrol was able to protect neuronal cells from isoflurane-induced inflammation and oxidative stress-associated death by attenuating apoptosis via Akt/p38 MAPK signaling.