Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco [Nicotiana tabacum] leaves

• Published in: Plant and cell physiology Vol. 39; no. 5; pp. 500 - 507
• Main Authors: Niki, T. (National Inst. of Agrobiological Resources, Tsukuba, Ibaraki (Japan)), Mitsuhara, I, Seo, S, Ohtsubo, N, Ohashi, Y
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-05-1998
• Subjects: ACIDE SALICYLIQUE; ACIDO SALICILICO; Agronomy. Soil science and plant productions; Biological and medical sciences; Chemical agents; disease resistance; Economic plant physiology; ESENCIA DE JAZMIN; ESSENCE DE JASMIN; EXPRESION GENICA; EXPRESSION DES GENES; FEUILLE; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; genes; genetic code; genetic regulation; Growth and development; Growth regulators; HERIDAS; HOJAS; inhibition; injuries; JASMINE OIL; Jasmonic acid; LEAVES; Molecular and cellular biology; Molecular genetics; NICOTIANA TABACUM; Patho-genesis related (PR) proteins; PATHOGENESE; PATHOGENESIS; pathogenesis-related proteins; PATOGENESIS; PLAIE; plant pathogens; Plant physiology and development; protein synthesis; Salicylic acid; SALICYLIC ACIDS; signals; stress response; Tobacco plant; Vegetative apparatus, growth and morphogenesis. Senescence; Wound signal transduction; WOUNDS; Terpenoid; Salicylic acid; Injury; Stimulant plant; Plant leaf; Gene expression; Nicotiana tabacum; Pathogenesis related protein; Signal transduction; Antagonism; Dicotyledones; Angiospermae; Plant growth substance; Defense mechanism; Spermatophyta; Phytohormone; Solanaceae
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029397

Salicylic acid (SA) and jasmonic acid (JA) are essential compounds in the pathogen- and wound-signaling pathways accompanying induced expression of acidic and basic pathogenesis-related (PR) protein genes, respectively. However, on the effect of exogenously supplied SA and JA in induction of PR gene expression, conflicting results have been obtained using various plant materials at different developmental stages. There is no clear evidence on these effects in the presence of both signals at the same time. We analyzed the effect of SA on wound- and JA-induced basic PR gene expression and that of Ja on SA-induced acidic PR gene expression in mature tobacco leaves. Wound-induced accumulation of transcripts for all 4 basic PR genes tested was enhanced in the presence of MeJA, and the inhibited in the presence of SA. On the other hand, expression of all 3 acidic PR genes tested was induced by SA and was inhibited by MeJA. Using antibodies raised against acidic PR-1 and PR-2 proteins, these effects were confirmed at the protein level. These results indicated that JA works as an inducer of basic PR genes, and also as an inhibitor for acidic PR genes, while SA does the opposite