Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

• Published in: Scientific data Vol. 7; no. 1; p. 404
• Main Authors: Li, Haoyuan, Nazari, Reza, Abbey, Brian, Alvarez, Roberto, Aquila, Andrew, Ayyer, Kartik, Barty, Anton, Berntsen, Peter, Bielecki, Johan, Pietrini, Alberto, Bucher, Maximilian, Carini, Gabriella, Chapman, Henry N., Contreras, Alice, Daurer, Benedikt J., DeMirci, Hasan, Flűckiger, Leonie, Frank, Matthias, Hajdu, Janos, Hantke, Max F., Hogue, Brenda G., Hosseinizadeh, Ahmad, Hunter, Mark S., Jönsson, H. Olof, Kirian, Richard A., Kurta, Ruslan P., Loh, Duane, Maia, Filipe R. N. C., Mancuso, Adrian P., Morgan, Andrew J., McFadden, Matthew, Muehlig, Kerstin, Munke, Anna, Reddy, Hemanth Kumar Narayana, Nettelblad, Carl, Ourmazd, Abbas, Rose, Max, Schwander, Peter, Marvin Seibert, M., Sellberg, Jonas A., Sierra, Raymond G., Sun, Zhibin, Svenda, Martin, Vartanyants, Ivan A., Walter, Peter, Westphal, Daniel, Williams, Garth, Xavier, P. Lourdu, Yoon, Chun Hong, Zaare, Sahba
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19-11-2020; Nature Publishing Group
• Subjects: 631/535; 639/624/1107/510; 639/766/747; BASIC BIOLOGICAL SCIENCES; Biological physics; Coliphages; Data Descriptor; Humanities and Social Sciences; Imaging and sensing; Lasers; multidisciplinary; Particle Accelerators; Science; Science (multidisciplinary); Structural biology; Virion; X-Ray Diffraction
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/s41597-020-00745-2

Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3
μ
m x 1.7
μ
m full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.
Measurement(s)
X-ray diffraction data
Technology Type(s)
free electron laser
Sample Characteristic - Organism
Enterobacteria phage PR772
Sample Characteristic - Location
contiguous United States of America
Machine-accessible metadata file describing the reported data:
https://doi.org/10.6084/m9.figshare.13070546