MiR-30e-5p deficiency exerts an inhibitory effect on inflammation in rheumatoid arthritis via regulating Atl2 expression

• Published in: Archives of rheumatology Vol. 38; no. 1; pp. 119 - 128
• Main Authors: Liu, Shanshan, Wang, Kai, Li, Ju, Liu, Yan, Zhang, Zhongyuan, Meng, Deqian
• Format: Journal Article
• Language: English
• Published: Istanbul Turkish League Against Rheumatism 01-03-2023; Prof Sebnem Ataman, President Turkish League Against Rheumatism
• Subjects: Arthritis; Binding sites; Enzyme-linked immunosorbent assay; Enzymes; Fibroblasts; Inflammation; Kinases; Laboratory animals; MicroRNA; MicroRNAs; Original; Polymerase chain reaction; Proteins; Rheumatoid arthritis; Rheumatoid factor; Software; Statistical analysis; Tumor necrosis factor-TNF; Variance analysis; China; United States > US
• ISSN: 2148-5046; 1309-0291; 2618-6500; 1309-0283
• DOI: 10.46497/ArchRheumatol.2023.9526

Objectives: This study aims to investigate the inflammatory effect of the microRNA (miRNA) miR-30e-5p on rheumatoid arthritis (RA) development in RA mice and fibroblast-like synoviocytes (FLS). Materials and methods: MiR-30e-5p and atlastin GTPase 2 (Atl2) expression in RA tissues and RA-FLS was evaluated using real-time quantitative polymerase chain reaction. The function of miR-30e-5p in inflammation of RA mice and RA-FLS was analyzed by enzyme- linked immunosorbent assay (ELISA) and Western blotting. 5-ethynyl-2'-deoxyuridine (EdU) assay was used to detect RA-FLS proliferation. Luciferase reporter assay was to confirm the interaction between miR-30e-5p and Atl2. Results: MiR-30e-5p expression was upregulated in the tissues from RA mice. Silencing miR-30e-5p alleviated inflammation in RA mice and RA-FLS. MiR-30e-5p negatively modulated Atl2 expression. Atl2 knockdown exerted a proinflammatory effect on RA-FLS. Atl2 knockdown rescued the inhibitory effect of miR-30e-5p knockdown on proliferation and inflammatory response of RA-FLS. Conclusion: MiR-30e-5p knockdown inhibited the inflammatory response in RA mice and RA-FLS through Atl2. Keywords: Atl2, fibroblast-like synoviocytes, inflammatory response, MiR-30e-5p, rheumatoid arthritis.