Impact of different resistance training protocols on muscular oxidative stress parameters

• Published in: Applied physiology, nutrition, and metabolism Vol. 37; no. 6; p. 1239
• Main Authors: Scheffer, Débora L, Silva, Luciano A, Tromm, Camila B, da Rosa, Guilherme L, Silveira, Paulo C L, de Souza, Claudio T, Latini, Alexandra, Pinho, Ricardo A
• Format: Journal Article
• Language: English
• Published: Canada 01-12-2012
• Subjects: Animals; Antioxidants - analysis; Antioxidants - metabolism; Catalase - analysis; Catalase - metabolism; Glutathione Peroxidase - analysis; Glutathione Peroxidase - metabolism; Glycogen - analysis; Lactic Acid - analysis; Male; Muscle, Skeletal - chemistry; Muscle, Skeletal - physiology; Oxidative Stress - physiology; Rats; Rats, Wistar; Resistance Training - methods; Superoxide Dismutase - analysis; Superoxide Dismutase - metabolism; Superoxides - metabolism; Thiobarbituric Acid Reactive Substances - analysis
• ISSN: 1715-5312
• DOI: 10.1139/h2012-115

This study analyzes oxidative stress in skeletal muscle using different resisted training protocols. We hypothesize that different types of training produce different specifics. To test our hypothesis, we defined 3 resistance training protocols and investigated the respective biochemical responses in muscle. Twenty-four male Wistar rats were distributed in 4 groups: untrained (UT), muscular resistance training (RT), hypertrophy training (HT), and strength training (ST). After 12 weeks of training on alternate days, the red portion of the brachioradialis was removed and the following parameters were assessed: lactate and glycogen content, superoxide production, antioxidant enzyme content, and activities (superoxide dismutase, SOD; catalase, CAT; GPx, glutathione peroxidase). Thiobarbituric acid-reactive substances (TBARS), carbonyl, and thiol groups were also measured. Results showed increased superoxide production (UT = 5.348 ± 0.889; RT = 5.117 ± 0,651; HT = 8.412 ± 0.431; ST = 6.354 ± 0.552), SOD (UT = 0.078 ± 0.0163; RT = 0.101 ± 0.013; HT = 0.533 ± 0.109; ST = 0.388 ± 0.058), GPx (UT = 0.290 ± 0.023; RT = 0.348 ± 0.014; HT = 0.529 ± 0.049; ST = 0.384 ± 0.038) activities, and content of GPx (HT = 3.8 times; ST = 3.0 times) compared with the UT group. CAT activity was lower (UT = 3.966 ± 0.670; RT = 3.474 ± 0.583; HT = 2.276 ± 0.302; ST = 2.028 ± 0.471) in HT and ST groups. Oxidative damage was observed in the HT group (TBARS = 0.082 ± 0.009; carbonyl = 0.73 ± 0.053; thiol = 12.78 ± 0.917) compared with the UT group. These findings indicate that HT causes an imbalance in oxidative parameters in favor of pro-oxidants, causing oxidative stress in skeletal muscle.