Cloning and structure of cDNA encoding α-latrotoxin from black widow spider venom

• Published in: FEBS letters Vol. 270; no. 1; pp. 127 - 131
• Main Authors: Kiyatkin, N.I., Dulubova, I.E., Chekhovskaya, I.A., Grishin, E.V.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 17-09-1990; Elsevier
• Subjects: Amino Acid Sequence; Amino acid sequence homology; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Biological and medical sciences; cDNA nucleotide sequence; Cloning, Molecular; Deduced primary structure; Dna, deoxyribonucleoproteins; Fundamental and applied biological sciences. Psychology; Gene Library; Molecular Sequence Data; Nucleic acids; Peptide Fragments - genetics; Poly A - genetics; Repetitive Sequences, Nucleic Acid; RNA, Messenger - genetics; Spider Venoms - chemistry; Spider Venoms - genetics; α-Latrotoxin precursor; Amino acid sequence homology; cDNA nucleotide sequence; Deduced primary structure; α-Latrotoxin precursor; Nucleotide sequence; Repeated sequence; Homology; Precursor; Open reading frame; Arachnida; Complementary DNA; Arthropoda; Venom; Restriction map; Invertebrata; Molecular cloning; Computer aid; Aminoacid sequence
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)81250-R

cDNA encoding the putative α-latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base-pair open reading frame corresponding to the 156 855-Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C-tenninal regions.