Lipidic Formulations Inspired by COVID Vaccines as Smart Coatings to Enhance Nanoparticle-Based Cancer Therapy

• Published in: Nanomaterials (Basel, Switzerland) Vol. 13; no. 15; p. 2250
• Main Authors: Conte, Marzia, Carofiglio, Marco, Rosso, Giada, Cauda, Valentina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-08-2023; MDPI
• Subjects: Antibodies; Biocompatibility; Biomedical materials; biomimetic nanoparticles; Cancer; Cancer therapies; Care and treatment; Cell viability; Cholesterol; Coatings; COVID-19 vaccines; Diagnosis; Drug delivery systems; Fluorescence; Gene therapy; Hyperthermia; Identification and classification; In vivo methods and tests; lipid nanoparticles; Lipids; Magnetic resonance imaging; Metal oxides; mRNA; mRNA vaccines; Nanoparticles; Nanotechnology; Pancreatic cancer; Peptides; phospholipid shell; Photodynamic therapy; Polyethylene glycol; Properties; solvent exchange method; Solvents; Vaccines; Zinc oxide; zinc oxide nanoparticles; Zinc oxides; Italy; United States > US; biomimetic nanoparticles; lipid nanoparticles; solvent exchange method; zinc oxide nanoparticles; phospholipid shell
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano13152250

Recent advances in nanomedicine have led to the introduction and subsequent establishment of nanoparticles in cancer treatment and diagnosis. Nonetheless, their application is still hindered by a series of challenges related to their biocompatibility and biodistribution. In this paper, we take inspiration from the recently produced and widely spread COVID vaccines, based on the combinational use of ionizable solid lipid nanoparticles, cholesterol, PEGylated lipids, and neutral lipids able to incorporate mRNA fragments. Here, we focus on the implementation of a lipidic formulation meant to be used as a smart coating of solid-state nanoparticles. The composition of this formulation is finely tuned to ensure efficient and stable shielding of the cargo. The resulting shell is a highly customized tool that enables the possibility of further functionalizations with targeting agents, peptides, antibodies, and fluorescent moieties for future in vitro and in vivo tests and validations. Finally, as a proof of concept, zinc oxide nanoparticles doped with iron and successively coated with this lipidic formulation are tested in a pancreatic cancer cell line, BxPC-3. The results show an astonishing increase in cell viability with respect to the same uncoated nanoparticles. The preliminary results presented here pave the way towards many different therapeutic approaches based on the massive presence of highly biostable and well-tolerated nanoparticles in tumor tissues, such as sonodynamic therapy, photodynamic therapy, hyperthermia, and diagnosis by means of magnetic resonance imaging.