Identification of ABF/AREB gene family in tomato ( Solanum lycopersicum L.) and functional analysis of ABF/AREB in response to ABA and abiotic stresses

• Published in: PeerJ (San Francisco, CA) Vol. 11; p. e15310
• Main Authors: Pan, Xuejuan, Wang, Chunlei, Liu, Zesheng, Gao, Rong, Feng, Li, Li, Ailing, Yao, Kangding, Liao, Weibiao
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 04-05-2023; PeerJ Inc
• Subjects: ABF/AREB; Abiotic stress; Abscisic acid; Abscisic Acid - pharmacology; Agricultural Science; Basic-Leucine Zipper Transcription Factors - genetics; Bioinformatics; Biotechnology; Cold-Shock Response; DNA binding proteins; Gene family analysis; Genes; Genomes; Genomics; Molecular Biology; Phylogeny; Plant Proteins - genetics; Plant Science; Plants - metabolism; Protein binding; Radiotherapy; Solanum lycopersicum - genetics; Tomato; Tomatoes; Transcription factor; China; ABF/AREB; Abscisic acid; Gene family analysis; Tomato; Transcription factor; Abiotic stress
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/PEERJ.15310

Abscisic acid (ABA) is a plant hormone that plays an important regulatory role in plant growth and stress response. The
(ABA-responsive element binding protein)/
(ABRE-binding factor) are important ABA-signaling components that participate in abiotic stress response. However, genome-scale analysis of
has not been systemically investigated in tomato. This study was conducted to identify tomato
family members and analyze their response to ABA and abiotic stresses. The results show that a total of 10
members were identified in tomato, which are randomly distributed on five chromosomes. Domain analysis showed that these members exhibit high protein similarity, especially in the basic leucine zipper (bZIP) domain region. Subcellular localization analysis indicated that all 10
members are localized in the nucleus. Phylogenetic tree analysis showed that tomato
genes are divided into two groups, and they are similar with the orthologs of other plants. The analysis of
-acting elements showed that most tomato
genes contain a variety of hormones and stress-related elements. Expression profiles of different tissues indicated that
and
play an important role in fruit ripening. Finally, qRT-PCR analysis revealed that 10 tomato
genes respond to ABA, with
being the most sensitive.
,
and
positively respond to salt and cold stresses.
,
and
are significantly induced under UV radiation treatment.
and
are significantly induced in osmotic stress. Overall, this study may provide insight into the role of tomato
homologues in plant response to abiotic stresses, which laid a foundation for future functional study of
in tomato.