Epidemiological characteristics of Salmonella Typhimurium isolated from animals and feed in Poland

• Published in: Epidemiology and infection Vol. 134; no. 1; pp. 179 - 185
• Main Authors: WASYL, D., SANDVANG, D., SKOV, M. N., BAGGESEN, D. L.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01-02-2006
• Subjects: Animal Feed - microbiology; Animals; Antibiotic resistance; Antimicrobials; Bacteriology; Bacteriophages; Biological and medical sciences; Drug Resistance, Microbial; Drug Resistance, Multiple; Epidemiologic Studies; Fundamental and applied biological sciences. Psychology; Humans; Infections; Microbiology; Miscellaneous; Plasmids; Poland - epidemiology; Poultry; Poultry Diseases - drug therapy; Poultry Diseases - epidemiology; Prevalence; Public Health; Risk Assessment; Salmonella; Salmonella enterica; Salmonella Infections, Animal - drug therapy; Salmonella Infections, Animal - epidemiology; Salmonella typhimurium; Salmonella typhimurium - classification; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Salmonella typhimurium - pathogenicity; Serotyping; Swine; Swine Diseases - drug therapy; Swine Diseases - epidemiology; Poland; Europe; Human; Bacteria; Microbiology; Epidemiology; Salmonella typhimurium; Enterobacteriaceae
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S0950268805004838

Fifty-seven Salmonella Typhimurium strains isolated from poultry, swine and animal feed in Poland during the years 1979–1998 and 2000–2002 were analysed with conventional and molecular techniques. Antimicrobial resistance as well as multiresistance was found, respectively, in 80·1% and 56·1% of the isolates and most frequently among isolates from 2000–2002. Of several phage types noted, DT104 was prevalent among poultry, swine and feed isolates. DT104, U302 and non-typable strains had a multiple resistant profile (ACSSuT) due to the presence of class I integrons. Pulse-field gel electrophoresis of XbaI and BlnI digest showed high genomic similarity between the strains and confirmed clonal spread of S. Typhimurium infections. Plasmid profiling allowed further differentiation of the strains. We have, therefore, confirmed the appearance of S. Typhimurium DT104 showing genome integrated integron-mediated antimicrobial resistance in Poland. These findings are significant for public and animal health risks and document the dissemination of DT104 epidemic strains into new geographical regions.