Comprehensive Cell Adhesion Analysis Using Electrochemiluminescence Imaging and Electrochemical Impedance Spectroscopy

• Published in: Denki kagaku oyobi kōgyō butsuri kagaku Vol. 92; no. 2; p. 022009
• Main Authors: OBA, Kimiharu, INO, Kosuke, UTAGAWA, Yoshinobu, ABE, Hiroya, SHIKU, Hitoshi
• Format: Journal Article
• Language: English
• Published: Tokyo The Electrochemical Society of Japan 06-02-2024; Japan Science and Technology Agency
• Subjects: Adhesion; Apoptosis; Cell adhesion; Cell adhesion & migration; Cell Analysis; Cell culture; Cellular Adhesion; Drug development; Electrochemical Impedance Spectroscopy; Electrochemiluminescence; Electrochemiluminescence Imaging; Electrochemistry; Endothelial cells; Extracellular matrix; Imaging; Impedance; Indium tin oxides; Spectroscopy; Spectrum analysis; Substrates
• ISSN: 1344-3542; 2186-2451
• DOI: 10.5796/electrochemistry.23-68109

Cell adhesion to culture substrates plays a crucial role in cellular activities, such as proliferation, differentiation, and apoptosis. Recently, electrochemiluminescence (ECL) imaging has been utilized for analyzing cell adhesion. In addition to ECL imaging, electrochemical impedance spectroscopy (EIS) is widely used as a conventional method for evaluating cell adhesion. However, the relationship between the results obtained from ECL imaging and EIS has not yet been investigated. In this study, the relationship between the results obtained using two methods is explored, and their advantages and disadvantages have been discussed. Endothelial cells were cultured on an extracellular matrix-coated indium tin oxide electrode for 24 h, which was further used for ECL imaging and EIS. To the best of our knowledge, this is the first report of ECL imaging and EIS of the same samples for cell adhesion analysis. This comprehensive analysis of cell adhesion using EIS and ECL imaging could be further used to evaluate drugs that target cell adhesion.