Pathological Relationship between Intracellular Superoxide Metabolism and p53 Signaling in Mice

• Published in: International journal of molecular sciences Vol. 22; no. 7; p. 3548
• Main Authors: Watanabe, Kenji, Shibuya, Shuichi, Ozawa, Yusuke, Toda, Toshihiko, Shimizu, Takahiko
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29-03-2021; MDPI
• Subjects: Age; Aging; Alzheimer's disease; Animals; Antioxidants; Apoptosis; Apoptosis - genetics; Atrophy; Cardiomyocytes; Cardiomyopathy; Cell activation; Cell cycle; Cell death; Cell growth; Cell viability; Degeneration; Dilated cardiomyopathy; DNA repair; Female; Fibroblasts; Fibroblasts - metabolism; Heart - physiopathology; Heart failure; Homeostasis; In vitro fertilization; Intracellular; Intracellular signalling; Male; Mice; Mice, Knockout; Mice, Transgenic; Mitochondria; Neoplasms - genetics; p53; p53 Protein; Phenotype; Rodents; Signal Transduction - genetics; Skin; superoxide; Superoxide dismutase; superoxide dismutase (SOD); Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase-1 - genetics; Superoxide Dismutase-1 - metabolism; Superoxides - metabolism; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Tumorigenesis; apoptosis; aging; superoxide; superoxide dismutase (SOD); p53
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22073548

Intracellular superoxide dismutases (SODs) maintain tissue homeostasis via superoxide metabolism. We previously reported that intracellular reactive oxygen species (ROS), including superoxide accumulation caused by cytoplasmic SOD (SOD1) or mitochondrial SOD (SOD2) insufficiency, induced p53 activation in cells. SOD1 loss also induced several age-related pathological changes associated with increased oxidative molecules in mice. To evaluate the contribution of p53 activation for SOD1 knockout (KO) (
) mice, we generated SOD1 and p53 KO (double-knockout (DKO)) mice. DKO fibroblasts showed increased cell viability with decreased apoptosis compared with
fibroblasts. In vivo experiments revealed that p53 insufficiency was not a great contributor to aging-like tissue changes but accelerated tumorigenesis in
mice. Furthermore, p53 loss failed to improve dilated cardiomyopathy or the survival in heart-specific SOD2 conditional KO mice. These data indicated that p53 regulated ROS-mediated apoptotic cell death and tumorigenesis but not ROS-mediated tissue degeneration in SOD-deficient models.