Structural Characterization and Osseointegrative Properties of Pulsed Laser-Deposited Fluorinated Hydroxyapatite Films on Nano-Zirconia for Implant Applications

• Published in: International journal of molecular sciences Vol. 23; no. 5; p. 2416
• Main Authors: Li, Min, Komasa, Satoshi, Hontsu, Shigeki, Hashimoto, Yoshiya, Okazaki, Joji
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22-02-2022; MDPI
• Subjects: Alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; Biological activity; Cells, Cultured; Coated Materials, Biocompatible - administration & dosage; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Coatings; Contact angle; Dental Implants; Dental materials; Dental prosthetics; Dental restorative materials; Durapatite - chemistry; Femur; Femur - cytology; Femur - drug effects; Femur - metabolism; Femur - surgery; fluorinated hydroxyapatite; Fluorination; Fluorine - chemistry; Gene expression; Hydroxyapatite; implant; Lasers; Male; Materials Testing; Mechanical properties; Mineralization; Morphology; nano-zirconia; Nanostructures; Osseointegration; Osseointegration - drug effects; Prostheses and Implants; Pulsed laser deposition; Pulsed lasers; Rats; Rats, Sprague-Dawley; Structural analysis; Surface Properties; Zirconia; Zirconium - administration & dosage; Zirconium - chemistry; Zirconium - pharmacology; Zirconium dioxide; implant; pulsed laser deposition; osseointegration; fluorinated hydroxyapatite; nano-zirconia
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23052416

Standard zirconia implants used in restoration still present problems related to inertness and long-term stability. Various physicochemical approaches have been used to modify the implant surfaces to improve early and late bone-to-implant integration; however, no ideal surface modification has been reported. This study used pulsed laser deposition to deposit a fluorinated hydroxyapatite (FHA) film on a zirconia implant to create a biologically active surface. The film prepared was uniform, dense, and crack-free, and exhibited granular surface droplets; it also presented excellent mechanical strength and favorable biological behavior. The FHA-coated implant was implanted on the femur of Sprague-Dawley rats, and various tests and analyses were performed. Results show that the in vitro initial cell activity on the FHA-coated samples was enhanced. In addition, higher alkaline phosphatase activity and cell mineralization were detected in cells cultured on the FHA-coated groups. Further, the newly formed bone volume of the FHA-coated group was higher than that of the bare micro-adjusted composite nano-zirconia (NANOZR) group. Therefore, the FHA film facilitated osseointegration and may improve the long-term survival rates of dental implants, and could become part of a new treatment technology for implant surfaces, promoting further optimization of NANOZR implant materials.