Comprehensive Targeted Metabolomic Study in the Lung, Plasma, and Urine of PPE/LPS-Induced COPD Mice Model

• Published in: International journal of molecular sciences Vol. 23; no. 5; p. 2748
• Main Authors: Kim, Hyeon-Young, Lee, Hyeon-Seong, Kim, In-Hyeon, Kim, Youngbae, Ji, Moongi, Oh, Songjin, Kim, Doo-Young, Lee, Wonjae, Kim, Sung-Hwan, Paik, Man-Jeong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02-03-2022; MDPI
• Subjects: Animals; Biomarkers; Biomarkers - metabolism; Chronic obstructive pulmonary disease; Discriminant analysis; Disease Models, Animal; Emphysema; Evaluation; Histidine; Inflammation; Isocitric acid; Lipopolysaccharides; Lipopolysaccharides - metabolism; Lung - metabolism; Lung diseases; Lungs; Mass spectrometry; Metabolism; Metabolites; Metabolomics; Mice; Neutrophils; Obstructive lung disease; Personal Protective Equipment; Phenylalanine; Physical characteristics; Plasma; potential biomarker; PPE/LPS-induced COPD exacerbation mice model; Pulmonary Disease, Chronic Obstructive - diagnosis; Pulmonary functions; Pyruvic acid; receiver operating characteristic analysis; Respiratory function; Scientific imaging; Succinic acid; Urine; PPE/LPS-induced COPD exacerbation mice model; metabolomics; potential biomarker; receiver operating characteristic analysis; chronic obstructive pulmonary disease
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23052748

(1) Background: Progression of chronic obstructive pulmonary disease (COPD) leads to irreversible lung damage and inflammatory responses; however, biomarker discovery for monitoring of COPD progression remains challenging. (2) Methods: This study evaluated the metabolic mechanisms and potential biomarkers of COPD through the integrated analysis and receiver operating characteristic (ROC) analysis of metabolic changes in lung, plasma, and urine, and changes in morphological characteristics and pulmonary function in a model of PPE/LPS-induced COPD exacerbation. (3) Results: Metabolic changes in the lungs were evaluated as metabolic reprogramming to counteract the changes caused by the onset of COPD. In plasma, several combinations of phenylalanine, 3-methylhistidine, and polyunsaturated fatty acids have been proposed as potential biomarkers; the α-aminobutyric acid/histidine ratio has also been reported, which is a novel candidate biomarker for COPD. In urine, a combination of succinic acid, isocitric acid, and pyruvic acid has been proposed as a potential biomarker. (4) Conclusions: This study proposed potential biomarkers in plasma and urine that reflect altered lung metabolism in COPD, concurrently with the evaluation of the COPD exacerbation model induced by PPE plus LPS administration. Therefore, understanding these integrative mechanisms provides new insights into the diagnosis, treatment, and severity assessment of COPD.