Bioshell Calcium Oxide (BiSCaO) Ointment for the Disinfection and Healing of Pseudomonas aeruginosa -Infected Wounds in Hairless Rats

• Published in: International journal of molecular sciences Vol. 21; no. 11; p. 4176
• Main Authors: Takayama, Tomohiro, Ishihara, Masayuki, Nakamura, Shingo, Sato, Yoko, Hiruma, Sumiyo, Fukuda, Koichi, Murakami, Kaoru, Yokoe, Hidetaka
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-06-2020; MDPI
• Subjects: Animal Shells - chemistry; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics; Bacteria; bactericidal activity; bioshell calcium oxide (BiSCaO); Calcium Compounds - chemistry; Calcium Compounds - pharmacology; Calcium oxide; Chronic infection; Cytokines; Disinfectants; Disinfection; Disinfection & disinfectants; Disinfection - methods; Drug resistance; Granulation; Growth factors; Hairless; infected wound; Infections; Iodine; Lime; Male; Microbicides; ointment; Ointments; Ointments - chemistry; Ointments - pharmacology; Oxides - chemistry; Oxides - pharmacology; Pectinidae - chemistry; Povidone; Povidone-Iodine - pharmacology; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas Infections - drug therapy; Pseudomonas Infections - pathology; Rats, Hairless; Ulcers; Wound healing; Wound Infection - drug therapy; Wound Infection - microbiology; Wound Infection - pathology; wound repair; Japan; bactericidal activity; wound repair; infected wound; ointment; bioshell calcium oxide (BiSCaO)
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21114176

Bioshell calcium oxide (BiSCaO) possesses deodorizing properties and broad microbicidal activity. This study aimed to investigate the application of BiSCaO ointment for the prevention and treatment of infection in chronic wounds in healing-impaired patients, without delaying wound healing. The bactericidal activities of 0.04, 0.2, 1, and 5 wt% BiSCaO ointment, 3 wt% povidone iodine ointment, and control (ointment only) were compared to evaluate the in vivo disinfection and healing of
-infected wounds in hairless rats. Treatment of the infected wounds with 0.2 wt% BiSCaO ointment daily for 3 days significantly enhanced wound healing and reduced the in vivo bacterial counts compared with povidone iodine ointment and control (no wound cleaning). Although 5 wt% BiSCaO ointment provided the lowest bacterial counts during 3 days' treatment, it delayed wound healing. Histological examinations showed significantly advanced granulation tissue and capillary formation in wounds treated with 0.2 wt% BiSCaO ointment for 3 days compared to wounds treated with the other ointments. This study suggested that using 0.2 wt% BiSCaO ointment as a disinfectant for infected wounds and limiting disinfection to 3 days may be sufficient to avoid the negative effects of BiSCaO on wound repair.