Heterogeneity of synaptic glutamate receptors on CA3 stratum radiatum interneurones of rat hippocampus

• Published in: The Journal of physiology Vol. 462; no. 1; pp. 373 - 392
• Main Authors: McBain, C J, Dingledine, R
• Format: Journal Article
• Language: English
• Published: Oxford The Physiological Society 01-03-1993; Blackwell
• Subjects: 2-Amino-5-phosphonovalerate - pharmacology; Animals; Biological and medical sciences; Cells, Cultured; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Fundamental and applied biological sciences. Psychology; Hippocampus - drug effects; Hippocampus - physiology; Interneurons - drug effects; Interneurons - physiology; Kainic Acid - metabolism; Rats; Rats, Sprague-Dawley; Receptors, Glutamate - physiology; Receptors, Neurotransmitter - physiology; Synapses - physiology; Tetrodotoxin - pharmacology; Vertebrates: nervous system and sense organs; Rat; Rodentia; Central nervous system; Electrophysiology; Glutamate receptor; Ionic channel; Interneuron; Kainate receptor; Vertebrata; AMPA receptor; Mammalia; Synaptic transmission; Kinetics; Membrane potential; Excitatory postsynaptic potential; Hippocampus; Brain (vertebrata)
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/jphysiol.1993.sp019560

1. Whole-cell recordings were made from interneurons located within CA3 stratum radiatum of neonate rat hippocampal slices.
All experiments were performed in the continued presence of tetrodotoxin (1 microM) and bicuculline (5 microM) to permit the
isolation of spontaneous miniature excitatory synaptic currents (mEPSCs). 2. Two distinct populations of interneurones were
identified based on current-voltage relations of kainate and the kinetic properties of spontaneous mEPSCs. These cell types
were classified as type I and type II interneurones. 3. The I-V relation of kainate in type I cells was linear or modestly
outwardly rectifying. Currents reversed polarity close to 0 mV. The kainate I-V relationship in type II interneurones was
strongly inwardly rectifying with little or no outward current passed at potentials up to +50 mV. 4. Spontaneous mEPSCs were
observed at a low frequency. At -70 mV mEPSCs received by type I interneurones had fast rise times (approximately 1 ms) and
decay time constants (approximately 5 ms) and were mediated by alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)
receptors. Miniature EPSCs on type I interneurones reversed polarity at approximately 0 mV. At +50 mV the kinetics of the
mEPSCs on type I interneurones were slowed and comprised both AMPA and N-methyl-D-aspartate (NMDA) receptor-mediated components
as revealed by their sensitivity to 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and D-2-amino-5-phosphonovaleric acid (D-APV).
5. The kinetics of spontaneous mEPSCs on type II cells were slower than their type I counterparts at -70 mV. Spontaneous mEPSCs
received by type II interneurones showed extreme inward rectification with no cells possessing fast events at +50 mV. In a
few cells slowly rising and slowly falling spontaneous mEPSCs were observed at positive holding potentials. These events were
abolished by D-APV and were therefore mediated solely by NMDA receptor activation. 6. Type I or type II interneurones filled
with Lucifer Yellow or biocytin possessed similar morphologies. Both cell types were typically large triangular cells with
three to six branching dendrites often possessing varicosities. The dendrites of these interneurones arborized throughout
strata radiatum, pyramidale, oriens and the molecular layer of the dentate gyrus. 7. In the majority of interneurones (both
type I and II) the rise times of individual mEPSCs were correlated with their half-width and decay time constant, suggesting
that the shape of the mEPSC is in part determined by the dendritic origin of the synaptic input.