The antennal transcriptome analysis and characterizations of odorant-binding proteins in Megachile saussurei (Hymenoptera, Megachilidae)

• Published in: BMC genomics Vol. 24; no. 1; p. 781
• Main Authors: Li, Wei-Zhen, Kang, Wen-Juan, Zhou, Jing-Jiang, Shang, Su-Qin, Shi, Shang-Li
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15-12-2023; BioMed Central; BMC
• Subjects: Acetates; Acetic acid; Alfalfa; Amino acids; Analysis; Animal behavior; Annotations; Antennae; Attractants; Bees; Binding; Binding proteins; Body parts; Conserved sequence; Cysteine; Divergence; Females; Genomics; Homology; Hymenoptera; Information processing; Insects; Ligands; Lysine; Medicago sativa; Megachile saussurei; Megachilidae; Molecular docking; Molecular modelling; Neural coding; Nucleotide sequence; Octan-3-one; Odorant-binding protein; Odorant-binding proteins; Odorants; Odors; Olfaction; Olfactory perception; Osmia cornuta; Phylogenetics; Phylogeny; Physiological aspects; Pollination; Protein binding; Proteins; Signal transduction; Transcriptome; Transcriptomes; China; Megachile saussurei; Antennae; Odorant-binding proteins; Medicago sativa; Transcriptome
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-023-09871-8

Odorant-binding proteins (OBPs) are essential in insect's daily behaviors mediated by olfactory perception. Megachile saussurei Radoszkowski (Hymenoptera, Megachilidae) is a principal insect pollinating alfalfa (Medicago sativa) in Northwestern China. The olfactory function have been less conducted, which provides a lot of possibilities for our research.
Our results showed that 20 OBPs were identified in total. Multiple sequence alignment analysis indicated MsauOBPs were highly conserved with a 6-cysteine motif pattern and all belonged to the classic subfamily, coding 113-196 amino acids and sharing 41.32%-99.12% amino acid identity with known OBPs of other bees. Phylogenetic analysis indicated there were certain homologies existed among MsauOBPs and most sequences were clustered with that of Osmia cornuta (Hymenoptera, Megachilidae). Expression analysis showed the identified OBPs were mostly enriched in antennae instead of other four body parts, especially the MsauOBP2, MsauOBP3, MsauOBP4, MsauOBP8, MsauOBP11 and MsauOBP17, in which the MsauOBP2, MsauOBP4 and MsauOBP8 presented obvious tissue-biased expression pattern. Molecular docking results indicated MsauOBP4 might be the most significant protein in recognizing alfalfa flower volatile 3-Octanone, while MsauOBP13 might be the most crucial protein identifying (Z)-3-hexenyl acetate. It was also found the lysine was a momentous hydrophilic amino acid in docking simulations.
In this study, we identified and analyzed 20 OBPs of M. saussurei. The certain homology existed among these OBPs, while some degree of divergence could also be noticed, indicating the complex functions that different MsauOBPs performed. Besides, the M. saussurei and Osmia cornuta were very likely to share similar physiological functions as most of their OBPs were clustered together. MsauOBP4 might be the key protein in recognizing 3-Octanone, while MsauOBP13 might be the key protein in binding (Z)-3-hexenyl acetate. These two proteins might contribute to the alfalfa-locating during the pollination process. The relevant results may help determine the highly specific and effective attractants for M. saussurei in alfalfa pollination and reveal the molecular mechanism of odor-evoked pollinating behavior between these two species.