Synthetic pH-Responsive Polymers for Protein Transduction

Synthetic pH-Responsive Polymers for Protein Transduction

A pH‐responsive, endosomal membrane disruptive, metabolite‐derived polyamide PP‐75 is developed to deliver the MBP‐Apoptin fusion protein, which induces tumor‐specific apoptosis into human osteogenic sarcoma Saos‐2 cells. The intracellular distribution and colocalization of MBP‐Apoptin‐AF647 (MA‐AF6...

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Bibliographic Details
Published in:Advanced materials (Weinheim) Vol. 21; no. 38-39; pp. 3910 - 3914
Main Authors: Liechty, William B., Chen, Rongjun, Farzaneh, Farzin, Tavassoli, Mahvash, Slater, Nigel K. H.
Format: Journal Article
Language:English
Published: Weinheim WILEY-VCH Verlag 19-10-2009
WILEY‐VCH Verlag
Subjects:
Apoptin
pH-responsive polymer
Protein delivery
Transduction
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Summary:A pH‐responsive, endosomal membrane disruptive, metabolite‐derived polyamide PP‐75 is developed to deliver the MBP‐Apoptin fusion protein, which induces tumor‐specific apoptosis into human osteogenic sarcoma Saos‐2 cells. The intracellular distribution and colocalization of MBP‐Apoptin‐AF647 (MA‐AF649) and PP‐75‐FITC provide strong evidence that PP‐75 both enhances uptake and facilitates cytoplasmic release of MBP‐Apoptin.
Bibliography:Universities UK organizations
ArticleID:ADMA200901733
ark:/67375/WNG-WGGD2L78-8
Gates Cambridge Trust
istex:1955C4CA6B6CD066DA35FE0207282D9F785D794E
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0935-9648
1521-4095
DOI:10.1002/adma.200901733