Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion

• Published in: Nature communications Vol. 14; no. 1; p. 2980
• Main Authors: Park, Jeong-Su, Kim, Jun-Hyeong, Soh, Won-Chang, Kim, Na-Young, Lee, Kyung-Sik, Kim, Chang-Hyun, Chung, Ik-Joo, Lee, Sunjae, Kim, Hye-Ran, Jun, Chang-Duk
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24-05-2023; Nature Publishing Group; Nature Portfolio
• Subjects: 14/19; 38/61; 49/61; 631/250; 631/80; 64/60; 96; 96/1; 96/31; Adipogenesis; Antigen-presenting cells; Antigens; Cell activation; Cell division; Cell Membrane; Cell survival; Cholesterol; Endocytosis; Fatty acids; Humanities and Social Sciences; Internalization; Lipids; Lymphocytes; Lymphocytes T; Metabolism; Microvilli; Molting; multidisciplinary; Plasma membranes; Receptors; Receptors, Antigen, T-Cell; Science; Science (multidisciplinary); Shedding; T cell receptors; T-Lymphocytes
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-38707-y

Although T cell activation is known to involve the internalization of the T cell antigen receptor (TCR), much less is known regarding the release of TCRs following T cell interaction with cognate antigen-presenting cells. In this study, we examine the physiological mechanisms underlying TCR release following T cell activation. We show that T cell activation results in the shedding of TCRs in T cell microvilli, which involves a combined process of trogocytosis and enzymatic vesiculation, leading to the loss of membrane TCRs and microvilli-associated proteins and lipids. Surprisingly, unlike TCR internalization, this event results in the rapid upregulation of surface TCR expression and metabolic reprogramming of cholesterol and fatty acid synthesis to support cell division and survival. These results demonstrate that TCRs are lost through trogocytic ‘molting’ following T cell activation and highlight this mechanism as an important regulator of clonal expansion.
Following interaction with antigen-presenting cells, T cell receptors (TCR) can be internalized via endocytosis. In contrast to this established mechanism, this study shows that T cell activation can be followed by TCR shedding, leading to enhanced TCR expression, metabolic activity and proliferation.