Development of S Haplotype-Specific Markers to Identify Genotypes of Self-Incompatibility in Radish ( Raphanus sativus L.)

• Published in: Plants (Basel) Vol. 13; no. 5; p. 725
• Main Authors: Heo, Seong-Ho, Kim, Su-Yeon, Mo, Suk-Yeon, Park, Han-Yong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-03-2024; MDPI
• Subjects: Amino acids; Breeding; Daikon; Gene expression; Genes; Genetic aspects; Genotypes; Glycoproteins; Haplotypes; Identification; Incompatibility; Kinases; MSA; Nucleotide sequence; Plant breeding; Proteins; radish (Rahpanus sativus L.); Radishes; Raphanus sativus; S haplotype; Self-incompatibility; Testing; South Korea; Japan; self-incompatibility; MSA; S haplotype; radish (Rahpanus sativus L.)
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13050725

Radish (
L.), a root vegetable belonging to the Brassicaceae family, is considered one of the representative crops displaying sporophytic self-incompatibility (SSI). The utilization of a self-incompatibility system in F
breeding can improve the efficiency of cross-combinations, leading to a reduction in breeding time and aiding in the development of novel F
varieties. The successful implementation of this system necessitates the rapid and accurate identification of S haplotypes in parental lines. In this study, we identified a total of nine S haplotypes among 22 elite radish lines through Sanger sequencing. Subsequently, we obtained sequences for showing a 95% similarity to nine S haplotypes, along with sequences identified by other researchers using BLAST. Following this, multiple sequence alignment (MSA) was conducted to identify
and
sequence similarities, as well as polymorphisms within the class I and II groups. Subsequently, S haplotype-specific marker sets were developed, targeting polymorphic regions of
and
alleles. These markers successfully amplified each of the nine S haplotypes. These markers will play a crucial role in the rapid and precise identification of parental S haplotypes in the radish F
breeding process, proving instrumental in the radish F
purity test.