One-step Production of Sterically Stabilized Anionic Nanoliposome Using Microfluidic Device

• Published in: Journal of Oleo Science Vol. 71; no. 4; pp. 515 - 522
• Main Authors: Ohanna Maria Menezes M. da Costa, Priscilla C. O. S. Firmino, Strapasson, Guilherme B., Lucimara G. de la Torre, Malfatti-Gasperini, Antonio A., Júnior, Severino Alves
• Format: Journal Article
• Language: English
• Published: Japan Japan Oil Chemists' Society 01-01-2022; Japan Science and Technology Agency
• Subjects: anionic liposomes; Drug Delivery Systems - methods; hydrodynamic focusing; Immune system; Lab-On-A-Chip Devices; Liposomes; Microfluidic devices; Microfluidics; Nanomedicine; Polydispersity; Production methods; stealth anionic liposomes; unilamellar liposome; stealth anionic liposomes; anionic liposomes; hydrodynamic focusing; unilamellar liposome; microfluidics
• ISSN: 1345-8957; 1347-3352
• DOI: 10.5650/jos.ess21309

Anionic liposomes (AL) are very attractive for nanomedicine and some formulations have already been launched for clinical development. Despite the excellent potential, their application presents two major challenges: laborious production methods and rapid degradation and elimination from blood by the immune system. In this work, we optimized the production of AL and its stealth form (SAL) using a onestep microfluidic process. We obtained unilamellar and near-monodisperse (< 10%) AL composed by the commercial composition (DMPC:DMPG) with mean size small as 53.7 nm, which is optimized for application in drug delivery. We also obtained SAL with similar characteristics using the microfluidic technique, overcoming the limitation of conventional methods where SAL presents high polydispersity (> 30%). This study demonstrates the great potential of the microfluidic technique for one-step production of stealth anionic nanoliposomes with controlled sizes and reproducible characteristics.