A surface protein−imprinted biosensor based on boronate affinity for the detection of anti−human immunoglobulin G

• Published in: Mikrochimica acta (1966) Vol. 189; no. 3; p. 106
• Main Authors: Liu, Zixuan, Yin, Zheng-Zhi, Cai, Wenrong, Wu, Datong, Li, Junyao, Kong, Yong
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-03-2022; Springer; Springer Nature B.V
• Subjects: Affinity; Analytical Chemistry; Biosensing Techniques; Biosensors; Borates - chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Detectors; Electrochemical Techniques; Ferricyanides - chemistry; Graphene; Humans; IgG antibody; Immunoglobulin G; Immunoglobulin G - analysis; Immunoglobulins; Imprinted polymers; Membrane Proteins - chemistry; Microengineering; Molecular Imprinting; Nanochemistry; Nanotechnology; Original Paper; Polymerization; Polypyrroles; Proteins; Selectivity; Molecularly imprinted polymers; Surface imprinting; Boronate affinity; Gold nanobipyramids; Anti-IgG
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-022-05204-w

A surface protein-imprinted biosensor was constructed on a screen-printed carbon electrode (SPCE) for the detection of anti-human immunoglobulin G (anti-IgG). The SPCE was successively decorated with aminated graphene (NH
2
-G) and gold nanobipyramids (AuNBs) for signal amplification. Then 4-mercaptophenylboric acid (4-MPBA) was covalently anchored to the surface of AuNBs for capturing anti-IgG template through boronate affinity binding. The decorated SPCE was then deposited with an imprinting layer generated by the electropolymerization of pyrrole. After removal of the anti-IgG template by the dissociation of the boronate ester in an acidic solution, three-dimensional (3D) cavities complementary to the anti-IgG template were formed in the imprinting layer of polypyrrole (PPy). The molecularly imprinted polymers (MIP)-based biosensor was used for the detection of anti-IgG, exhibiting a wide linear range from 0.05 to 100 ng mL
−1
and a low limit of detection of 0.017 ng mL
−1
(S/N = 3). In addition, the MIP-based anti-IgG biosensor also shows high selectivity, reproducibility and stability. Finally, the practicability of the fabricated anti-IgG biosensor was demonstrated by accurate determination of anti-IgG in serum sample.