Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma

• Published in: Cell death & disease Vol. 12; no. 11; pp. 1063 - 11
• Main Authors: Li, Ce, Li, Wenming, Cao, Shengda, Xu, Jianing, Qian, Ye, Pan, Xinliang, Lei, Dapeng, Wei, Dongmin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09-11-2021; Springer Nature B.V; Nature Publishing Group
• Subjects: 13/109; 13/89; 38/1; 45/41; 45/77; 631/67/1536; 64/60; 692/699/67/1536; 82/51; Animals; Antibodies; beta Catenin - metabolism; Biochemistry; Bioinformatics; Biomedical and Life Sciences; c-Myc protein; Cancer; Carcinogenesis; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Cell Biology; Cell Culture; Cell Proliferation; Circular RNA; Epithelial-Mesenchymal Transition; Genes, myc - immunology; Humans; Hypopharyngeal Neoplasms - genetics; Hypopharyngeal Neoplasms - pathology; Immunology; Life Sciences; Metastases; Metastasis; Mice; MicroRNAs - metabolism; Myc protein; Neoplasm Metastasis; Squamous cell carcinoma; Throat cancer; Transfection; Tumorigenesis; β-Catenin
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-021-04346-8

Hypopharyngeal squamous cell carcinoma (HSCC) accounts 95% of hypopharyngeal cancer, which is characterized by high early metastasis rate and poor prognosis. It is reported that circular RNA is involved in the occurrence and development of cancer; however, the role of circRNA in hypopharyngeal cancer has little been investigated. We performed hypopharyngeal carcinoma circRNA microarray and qRT-PCR verification. The results showed circ_0058106 expression level was significantly upregulated in tumor tissues than in corresponding normal tissues. We found that circ_0058106 upregulation promoted proliferation, migration and invasion of HSCC cells, while knockdown of circ_0058106 inhibited proliferation, migration and invasion of HSCC cells both in vitro and in vivo. Bioinformatics predicted circ_0058106 may interact with miR-185-3p. We verified circ_0058106 directly bound miR-185-3p and downregulated miR-185-3p expression by using dual-luciferase reporter assay and qRT-PCR. Moreover, we proved circ_0058106 promoted HSCC cells tumorigenesis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway via miR-185-3p. In conclusion, our findings firstly confirmed the carcinogenic effect of circ_0058106 in promoting HSCC cells tumorigenesis, metastasis, invasion and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through sponging miR-185-3p, indicating that circ_0058106 may be a new therapeutic target and prognostic marker for HSCC.