Polarized NHE1 and SWELL1 regulate migration direction, efficiency and metastasis

• Published in: Nature communications Vol. 13; no. 1; pp. 6128 - 17
• Main Authors: Zhang, Yuqi, Li, Yizeng, Thompson, Keyata N., Stoletov, Konstantin, Yuan, Qinling, Bera, Kaustav, Lee, Se Jong, Zhao, Runchen, Kiepas, Alexander, Wang, Yao, Mistriotis, Panagiotis, Serra, Selma A., Lewis, John D., Valverde, Miguel A., Martin, Stuart S., Sun, Sean X., Konstantopoulos, Konstantinos
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17-10-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 13/109; 13/44; 13/51; 13/62; 14/1; 14/19; 14/33; 14/34; 14/35; 14/5; 14/63; 42/41; 42/44; 45/77; 45/88; 49; 631/67/1347; 631/67/322; 631/80/84/2336; 631/80/85; 64/60; 9/74; Breast cancer; Cancer; Cdc42 protein; Cell activation; Cell adhesion & migration; Cell migration; Cell Movement - physiology; Cell Size; Chloride ions; Extravasation; Humanities and Social Sciences; Humans; Ion channels; Ion exchangers; Leading edges; Metastases; Metastasis; multidisciplinary; Neoplasms; Physiology; Polarization; RhoA protein; Science; Science (multidisciplinary); Sodium-Hydrogen Exchangers; Spheroids; Trailing edges; Water; Water uptake
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-33683-1

Cell migration regulates diverse (patho)physiological processes, including cancer metastasis. According to the Osmotic Engine Model, polarization of NHE1 at the leading edge of confined cells facilitates water uptake, cell protrusion and motility. The physiological relevance of the Osmotic Engine Model and the identity of molecules mediating cell rear shrinkage remain elusive. Here, we demonstrate that NHE1 and SWELL1 preferentially polarize at the cell leading and trailing edges, respectively, mediate cell volume regulation, cell dissemination from spheroids and confined migration. SWELL1 polarization confers migration direction and efficiency, as predicted mathematically and determined experimentally via optogenetic spatiotemporal regulation. Optogenetic RhoA activation at the cell front triggers SWELL1 re-distribution and migration direction reversal in SWELL1-expressing, but not SWELL1-knockdown, cells. Efficient cell reversal also requires Cdc42, which controls NHE1 repolarization. Dual NHE1/SWELL1 knockdown inhibits breast cancer cell extravasation and metastasis in vivo, thereby illustrating the physiological significance of the Osmotic Engine Model.
Cell migration regulates diverse (patho)physiological processes, including cancer metastasis. Here the authors show that the chloride ion channel SWELL1 and the ion exchanger NHE1 are preferentially enriched at the trailing and leading edges, respectively, of migrating cells and regulate cell volume to propel confined cells, favouring breast cancer cell extravasation and metastasis.