Myocardial Injury After Ischemia/Reperfusion Is Attenuated By Pharmacological Galectin-3 Inhibition

• Published in: Scientific reports Vol. 9; no. 1; pp. 9607 - 10
• Main Authors: Ibarrola, Jaime, Matilla, Lara, Martínez-Martínez, Ernesto, Gueret, Alexandre, Fernández-Celis, Amaya, Henry, Jean-Paul, Nicol, Lionel, Jaisser, Frederic, Mulder, Paul, Ouvrard-Pascaud, Antoine, López-Andrés, Natalia
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03-07-2019; Nature Publishing Group
• Subjects: 13/51; 59/57; 631/337; 692/4019/592/75/230; 82/80; 96/21; Animals; Biomarkers; Blood flow; Compliance; Coronary artery; Disease Models, Animal; Extracellular matrix; Fibrosis; Galectin 3 - antagonists & inhibitors; Galectin 3 - genetics; Galectin-3; Gene Expression; Heart Failure - diagnosis; Heart Failure - drug therapy; Heart Failure - etiology; Heart Failure - metabolism; Heart Function Tests; Hemodynamics; Humanities and Social Sciences; Hypertrophy; Immunohistochemistry; Inflammation; Ischemia; Magnetic Resonance Imaging; multidisciplinary; Muscle contraction; Myocardial infarction; Myocardial Infarction - diagnosis; Myocardial Infarction - drug therapy; Myocardial Infarction - etiology; Myocardial Infarction - metabolism; Myocardial Reperfusion Injury - drug therapy; Myocardial Reperfusion Injury - etiology; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - pathology; Myocardium - metabolism; Myocardium - pathology; Pectin; Pectins - pharmacology; Perfusion; Rats; Reperfusion; Science; Science (multidisciplinary); Ventricle
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-46119-6

Although optimal therapy for myocardial infarction includes reperfusion to restore blood flow to the ischemic region, ischemia/reperfusion (IR) also initiates an inflammatory response likely contributing to adverse left ventricular (LV) extracellular matrix (ECM) remodeling. Galectin-3 (Gal-3), a β-galactoside-binding-lectin, promotes cardiac remodeling and dysfunction. Our aim is to investigate whether Gal-3 pharmacological inhibition using modified citrus pectin (MCP) improves cardiac remodeling and functional changes associated with IR. Wistar rats were treated with MCP from 1 day before until 8 days after IR (coronary artery ligation) injury. Invasive hemodynamics revealed that both LV contractility and LV compliance were impaired in IR rats. LV compliance was improved by MCP treatment 8 days after IR. Cardiac magnetic resonance imaging showed decreased LV perfusion in IR rats, which was improved with MCP. There was no difference in LV hypertrophy in MCP-treated compared to untreated IR rats. However, MCP treatment decreased the ischemic area as well as Gal-3 expression. Gal-3 blockade paralleled lower myocardial inflammation and reduced fibrosis. These novel data showing the benefits of MCP in compliance and ECM remodeling in IR reinforces previously published data showing the therapeutic potential of Gal-3 inhibition.