Alveolar bone repair of rhesus monkeys by using BMP-2 gene and mesenchymal stem cells loaded three-dimensional printed bioglass scaffold

• Published in: Scientific reports Vol. 9; no. 1; pp. 18175 - 12
• Main Authors: Wang, Liyan, Xu, Weikang, Chen, Yang, Wang, Jingjing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03-12-2019; Nature Publishing Group
• Subjects: 13/107; 42/100; 639/301/54/992; 639/301/54/993; Alveolar bone; Animals; Bone and Bones - metabolism; Bone and Bones - physiology; Bone Diseases - metabolism; Bone Diseases - therapy; Bone growth; Bone healing; Bone marrow; Bone morphogenetic protein 2; Bone Morphogenetic Protein 2 - metabolism; Bone Regeneration - physiology; Bones; Cell Proliferation - physiology; Ceramics - pharmacology; Computed tomography; Female; Humanities and Social Sciences; Macaca mulatta; Mesenchymal stem cells; Mesenchymal Stem Cells - metabolism; Mesenchyme; Monkeys & apes; multidisciplinary; Osteogenesis; Osteogenesis - physiology; Printing, Three-Dimensional; Regenerative medicine; Science; Science (multidisciplinary); Stem cell transplantation; Stem cells; Surgery; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds; Trauma; X-Ray Microtomography - methods
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-54551-x

Over the past years, the study about bone tissue engineering in the field of regenerative medicine has been a main research topic. Using three-dimensional (3D) porous degradable scaffold complexed with mesenchymal stem cells (MSCs) and growth factor gene to improve bone tissue repair and regeneration has raised much interest. This study mainly evaluated the osteogenesis of alveolar bone defects of animal in the following experimental groups: sham-operated (SO), 3D printed bioglass (3D-BG), 3D-BG with BMP-2 gene loaded CS (3D-BG + BMP/CS) and 3D-BG with rhesus marrow bone MSCs and BMP/CS (3D-BG + BMP/CS + rBMSCs). Simulated human bone defect with critical size of 10 × 10 × 5 mm were established in quadrumana - rhesus monkeys, and in
vivo
osteogenesis was characterized by X-ray, micro-Computed Tomography (mCT) and history. Our results revealed that 3D-BG + rBMSCs + BMP/CS scaffold could improve bone healing best by showing its promote osteogenic properties
in vivo
. Considering the great bone repair capacity of 3D-BG + BMP/CS + rBMSCs in humanoid primate rhesus monkeys, it could be a promising therapeutic strategy for surgery trauma or accidents, especially for alveolar bones defects.