Pre- and peri-implantation Zika virus infection impairs fetal development by targeting trophectoderm cells

• Published in: Nature communications Vol. 10; no. 1; pp. 4155 - 12
• Main Authors: Tan, Lei, Lacko, Lauretta A., Zhou, Ting, Tomoiaga, Delia, Hurtado, Romulo, Zhang, Tuo, Sevilla, Ana, Zhong, Aaron, Mason, Christopher E, Noggle, Scott, Evans, Todd, Stuhlmann, Heidi, Schwartz, Robert E., Chen, Shuibing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13-09-2019; Nature Publishing Group; Nature Portfolio
• Subjects: 13/100; 13/2; 14/19; 38/39; 49; 59; 631/136/1455; 631/326/596/2555; 64; 64/60; 692/308/575; 96/63; Abortion, Spontaneous - metabolism; Abortion, Spontaneous - physiopathology; Animals; Blastocyst - cytology; Blastocyst - metabolism; Cell death; Cells (biology); Embryo Implantation - physiology; Embryos; Female; Fetal Development - genetics; Fetal Development - physiology; Fetuses; Humanities and Social Sciences; Humans; Implantation; Infections; Mice; Mice, Inbred C57BL; Microencephaly; Miscarriage; multidisciplinary; Nervous system; Pregnancy; Pregnancy Complications, Infectious - metabolism; Progenitor cells; Ribonucleic acid; RNA; RNA, Viral - genetics; Science; Science (multidisciplinary); Transcription; Translational Medical Research - methods; Trophectoderm; Trophoblasts - cytology; Trophoblasts - metabolism; Vector-borne diseases; Viruses; Zika virus; Zika Virus - pathogenicity; Zika Virus Infection - complications; Zika Virus Infection - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-12063-2

Zika virus (ZIKV) infection results in an increased risk of spontaneous abortion and poor intrauterine growth although the underlying mechanisms remain undetermined. Little is known about the impact of ZIKV infection during the earliest stages of pregnancy, at pre- and peri-implantation, because most current ZIKV pregnancy studies have focused on post-implantation stages. Here, we demonstrate that trophectoderm cells of pre-implantation human and mouse embryos can be infected with ZIKV, and propagate virus causing neural progenitor cell death. These findings are corroborated by the dose-dependent nature of ZIKV susceptibility of hESC-derived trophectoderm cells. Single blastocyst RNA-seq reveals key transcriptional changes upon ZIKV infection, including nervous system development, prior to commitment to the neural lineage. The pregnancy rate of mice is >50% lower in pre-implantation infection than infection at E4.5, demonstrating that pre-implantation ZIKV infection leads to miscarriage. Cumulatively, these data elucidate a previously unappreciated association of pre- and peri-implantation ZIKV infection and microcephaly.
Here, using human cells and mouse models, the authors show that Zika virus can infect preimplantation trophectoderm. Pre-implantation infection can affect nervous system development and survival of neural progenitors, and can result in miscarriage or spontaneous abortion.