Probing the antioxidant activity of functional proteins and bioactive peptides in Hermetia illucens larvae fed with food wastes

• Published in: Scientific reports Vol. 12; no. 1; p. 2799
• Main Authors: Lu, Jiaxin, Guo, Yuwen, Muhmood, Atif, Zeng, Bei, Qiu, Yizhan, Wang, Pan, Ren, Lianhai
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18-02-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 704/158; 704/172; Animals; Antioxidants; Antioxidants - pharmacology; ATP synthase; Bioconversion; Chitin; Diptera - genetics; Diptera - metabolism; DNA helicase; Food; Food - adverse effects; Food Safety; Food waste; Fumarase; Glutamate dehydrogenase; Glycolysis; Hermetia illucens; Histone H2A; Humanities and Social Sciences; Larva - genetics; Larva - metabolism; Larvae; multidisciplinary; NADP; Peptides; Peptides - genetics; Peritrophin; Proteins; Proteins - genetics; Proteomics; Proteomics - methods; Refuse Disposal; Ribosomal protein L8; Ribosomal protein S3; Ribosomal protein S4; Ribosomal proteins; RNA helicase; Science; Science (multidisciplinary); Solid Waste; Wastes; Yeast
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-06668-9

Food waste is becoming more prevalent, and managing it is one of the most important issues in terms of food safety. In this study, functional proteins and bioactive peptides produced from the enzymatic digestion of black soldier fly (
Hermetia illucens L.
, BSF) fed with food wastes were characterized and quantified using proteomics-based analysis. The results revealed approximately 78 peptides and 57 proteins, including 40S ribosomal protein S4, 60S ribosomal protein L8, ATP synthase subunit alpha, ribosomal protein S3, Histone H2A, NADP-glutamate dehydrogenase, Fumarate hydratase, RNA helicase, Chitin binding Peritrophin-A, Lectin C-type protein, etc. were found in BSF. Furthermore, functional analysis of the proteins revealed that the 60S ribosomal protein L5 (RpL5) in BSF interacted with a variety of ribosomal proteins and played a key role in the glycolytic process (AT14039p). Higher antioxidant activity was found in peptide sequences such as GYGFGGGAGCLSMDTGAHLNR, VVPSANRAMVGIVAGGGRIDKPILK, AGLQFPVGR, GFKDQIQDVFK, and GFKDQIQDVFK. It was concluded that the bioconversion of food wastes by BSF brought about the generation of a variety of functional proteins and bioactive peptides with strong antioxidant activity. However, more studies are required to exploit BSF's potential in the value addition of food wastes.