Non-lethal effects of entomopathogenic nematode infection

• Published in: Scientific reports Vol. 11; no. 1; p. 17090
• Main Authors: Filgueiras, Camila C., Willett, Denis S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24-08-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158/853; 631/158/856; 631/250/2499; Animals; Biological control; Developmental stages; Diptera - growth & development; Diptera - parasitology; Entomopathogenic nematodes; Host-Parasite Interactions; Humanities and Social Sciences; Infections; Insects; Lethal effects; Mortality; Mortality risk; multidisciplinary; Nematoda - classification; Nematoda - pathogenicity; Nematodes; Parasites; Pest control; Pest Control, Biological - methods; Pests; Pupa - parasitology; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-96270-2

Entomopathogenic nematodes are typically considered lethal parasites of insect hosts. Indeed they are employed as such for biological control of insect pests. The effects of exposure to entomopathogenic nematodes are not strictly limited to mortality, however. Here we explore non-lethal effects of exposure to entomopathogenic nematodes by introducing the relatively non-susceptible pupal stage of
Delia antiqua
to thirteen different strains. We specifically chose to inoculate the pupal stage because it tends to be more resistant to infection, yet resides in the soil where it could come into contact with EPN biological control agents. We find that there is no significant mortality at the pupal stage, but that there are a host of strain-dependent non-lethal effects during and after the transition to adulthood including altered developmental times and changes in risk of death compared to controls. We also find that exposure to specific strains can reduce risk of mortality. These results emphasize the strain-dependent nature of entomopathogenic nematode infection and highlight the positive and negative ramifications for non-lethal effects for biological control of insect pests. Our work emphasizes the need for strain-specific screening of biological control agents before wide-spread adoption.