Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology

• Published in: Scientific reports Vol. 12; no. 1; p. 6736
• Main Authors: Yu, Ning, Liang, Yaping, Wang, Qingping, Peng, Xinxiang, He, Zhenghui, Hou, Xuewen
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25-04-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/337; 631/449; Gene Expression Profiling; Gene Expression Regulation, Plant; Genotype & phenotype; Humanities and Social Sciences; Leaves; multidisciplinary; Oryza - genetics; Oryza - metabolism; Phenotypes; Plant Leaves - metabolism; Rice; Science; Science (multidisciplinary); Signal transduction; Sunlight; Transcriptome; Transcriptomics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-10784-x

Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent
OsRUS1-GFP
overexpression (
OsRUS1-OX
) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was utilized to identify differentially expressed genes between flag leaves of
OsRUS1-OX
and wildtype under sunlight. 2920 genes were differentially expressed between
OsRUS1-OX
and WT, of which 1660 upregulated and 1260 downregulated. Six of the 16 genes in GO: 0009415 (response to water stimulus) were significantly upregulated in
OsRUS1-OX
. The differentially expressed genes between WT and
OsRUS1-OX
were assigned to 110 KEGG pathways. 42 of the 222 genes in KEGG pathway dosa04075 (Plant hormone signal transduction) were differentially expressed between WT and
OsRUS1-OX
. The identified genes in GO:0009415 and KEGG pathway dosa04075 were good candidates to explain the leaf rolling phenotype of
OsRUS1-OX
. The expression patterns of the 15 genes identified by RNA-Seq were verified by qRT-PCR. Based on transcriptomic and qRT-PCR analysis, a mechanism for the leaf rolling phenotype of
OsRUS1-OX
was proposed. The differential expression profiles between WT and
OsRUS1-OX
established by this study provide important insights into the molecular mechanism behind the leaf rolling phenotype of
OsRUS1-OX
.