Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs)

• Published in: AMB Express Vol. 11; no. 1; p. 60
• Main Authors: Yang, Cheng, Shao, Wangfeng, Wei, Lingling, Chen, Lingxiao, Zhu, Aihua, Pan, Zhiming
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 24-04-2021; Springer Nature B.V; SpringerOpen
• Subjects: Biomedical and Life Sciences; Biotechnology; Breeding; Clustered regularly interspaced short palindromic repeats; CRISPR; Dogs; Genes; Hospitals; Life Sciences; Microbial Genetics and Genomics; Microbiology; Molecular subtyping; Multilocus sequence typing; Original; Original Article; Pet; Pets; Salmonella; Salmonellosis; Serotypes; Kentucky; Multilocus sequence typing; Clustered regularly interspaced short palindromic repeats; Molecular subtyping; Pet; Salmonella
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-021-01221-9

Salmonella
, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog
Salmonella
from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype
Salmonella
isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples.
S.
Kentucky (40.74%) was the most prevalent serotype, but other, such as
S.
Typhimurium (18.52%) and
S.
Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of
Salmonella
genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of
Salmonella
and the prevention and treatment of salmonellosis.